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AB213682

Anti-Factor D/CFD antibody

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(3 Publications)

Rabbit Polyclonal Factor D/CFD antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFD aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DF, PFD, CFD, Complement factor D, Adipsin, C3 convertase activator, Properdin factor D

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor D/CFD antibody (AB213682)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor D/CFD antibody (AB213682)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling Factor D/CFD with ab213682 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CFD aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P00746

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Alternative Products

Primary Antibodies

AB36383

Anti-Factor D/CFD antibody [I8/1]

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Primary Antibodies

AB213177

Anti-Factor D/CFD antibody [EPR17004-240]

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Western blot - Anti-Factor D/CFD antibody [EPR17004-240] (AB213177)

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Primary Antibodies

AB181307

Anti-Factor D/CFD antibody [EPR17004-285]

RabMAb|Recombinant

Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (AB181307)

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Primary Antibodies

AB204917

Anti-Factor D/CFD antibody [EPR14699]

RabMAb|Recombinant

Western blot - Anti-Factor D/CFD antibody [EPR14699] (AB204917)

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Complementary Products

Assay Kits

AB243686

Human Complement Factor D ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB205198

Anti-AACT antibody [EPR17088-68]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR17088-68] (AB205198)

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Primary Antibodies

AB108310

Anti-CD13 antibody [EPR4058]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (AB108310)

  • 4
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Assay Kits

AB243686

Human Complement Factor D ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.
Biological function summary

Factor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.

Pathways

Factor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.

Abnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324, PubMed : 9748277). In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3 (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324). The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324). Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway (PubMed : 21205667, PubMed : 6769474, PubMed : 874324, PubMed : 9748277).
See full target information CFD
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 46:1759-1770 PubMed37188751

2023

Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Zhe Zhang,Yuan Li,Ying Zhang,Min Wei,Hui Li,Shanzhi Yang,Yali Zhou,Xinjin Zhou,Guolan Xing

Frontiers in cell and developmental biology 10:1044198 PubMed36420138

2022

The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Jingmei Ma,Yawei Liu,Zhirong Guo,Run Sun,Xinrui Yang,Weiran Zheng,Yongdan Ma,Yin Rong,Hongmei Wang,Huixia Yang,Zhenyu Xiao

RNA biology :1-12 PubMed33151124

2020

Gastrodin promotes CNS myelination via a lncRNA Gm7237/miR-142a/MRF pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Shu,Tianyuan Luo,Mingda Wang,Yu Zhang,Lin Zhang,Zhi Xiao,Qianxing Wang,Qiang Zhang,Jia Zou,Changyin Yu,Shangfu Xu,Tian Yu,Liang Zhou,Shouyang Yu
View all publications
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Product promise

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