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AB115290

Anti-Factor H antibody

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(1 Publication)

Goat Polyclonal Factor H antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CFH aa 550-600.

View Alternative Names

HF, HF1, HF2, CFH, Complement factor H, H factor 1

2 Images
Western blot - Anti-Factor H antibody (AB115290)
  • WB

Unknown

Western blot - Anti-Factor H antibody (AB115290)

All lanes:

Western blot - Anti-Factor H antibody (ab115290) at 0.03 µg/mL

All lanes:

Human lung lysate at 35 µg

Predicted band size: 139 kDa

Observed band size: 170 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody (AB115290)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody (AB115290)

Formalin-fixed, paraffin-embedded Human liver tissue labelled with ab115290 at 2.5 μg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CFH aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

P08603

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Alternative Products

Primary Antibodies

AB170036

Anti-Factor H antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody (AB170036)

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AB121055

Anti-Factor H antibody [C18/3]

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Primary Antibodies

AB118820

Anti-Factor H antibody [OX-24]

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Primary Antibodies

AB124769

Anti-Factor H antibody [EPR6226]

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Primary Antibodies

AB133536

Anti-Factor H antibody [EPR6225]

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Western blot - Anti-Factor H antibody [EPR6225] (AB133536)

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Complementary Products

Assay Kits

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Secondary Antibodies

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Primary Antibodies

AB305137

Anti-CFHR1 antibody [EPR25757-16] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR1 antibody [EPR25757-16] - BSA and Azide free (Detector) (AB305137)

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Primary Antibodies

AB309149

Anti-Complement factor B antibody [EPR27443-6]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Complement factor B antibody [EPR27443-6] (AB309149)

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Primary Antibodies

AB87424

Anti-C4d antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody (AB87424)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 98% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed : 21285368, PubMed : 21317894, PubMed : 25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed : 19503104, PubMed : 21317894, PubMed : 26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed : 18252712, PubMed : 23332154, PubMed : 28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed : 20008295, PubMed : 9558116).. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
See full target information CFH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 12:1580-1586 PubMed34381563

2021

High CD39 expression is associated with the non-muscle-invasive phenotype of human bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Janaina Mendes Ferreira,Luiz Henrique Gomes Matheus,Renato Vasconcelos Souza de Almeida,Petronio Augusto de Souza Melo,Kátia Ramos Moreira Leite,Claudio Bovolenta Murta,Joaquim Francisco de Almeida Claro,Cleber Pinto Camacho,José Pontes-Júnior,Humberto Dellê
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