Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)

Rabbit Recombinant Monoclonal Factor H antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (AB315880), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB	IHC-Fr	IP	Flow Cyt
Human	Tested	Expected	Tested	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Tested	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information Cfh

Function

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed.

Alternative names

Hf1, Cfh, Complement factor H, Protein beta-1-H

Recommended products

Rabbit Recombinant Monoclonal Factor H antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR26491-30
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for rat ICC.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315880 is the carrier-free version of Anti-Factor H antibody [EPR26491-30] ab315879.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.

Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Associated diseases and disorders

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Factor H is a glycoprotein of approximately 160 kDa and detected as a 140-kDa band after treated with Peptide:N-glycosidase F (PNGase F).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Factor H antibody [EPR26491-30] (Anti-Factor H antibody [EPR26491-30] ab315879) at 1/1000 dilution
Lane 1: Untreated Mouse liver tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate treated with Peptide: N-glycosidase F (PNGase F) at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 160 kDa, 140 kDa, 124 kDa
Exposure time: 180s
Western blot - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Samples are non-boiled as boiling may cause protein aggregation.
Lanes 1 and 3 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lane 2 is applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.
Exposure time: Lane 1: 6 seconds, lanes 2-3: 15 seconds.
All lanes: Western blot - Anti-Factor H antibody [EPR26491-30] (Anti-Factor H antibody [EPR26491-30] ab315879) at 1/1000 dilution
Lane 1: Rat plasma lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human plasma lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse plasma lysate at 20 µg with 5% NFDM/TBST
Secondary
Lanes 1 and 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 160 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L-929 (mouse connective tissue fibroblast) cells labelling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in L-929 cells treated with IFN-gamma (500 units/ml) and dexamethasone (10 μM) for 24 hr. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Western blot - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Low expression: cerebellum.
Samples are non-boiled as boiling may cause protein aggregation.
Lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-3: 203 seconds, lanes 4-5: 37 seconds.
All lanes: Western blot - Anti-Factor H antibody [EPR26491-30] (Anti-Factor H antibody [EPR26491-30] ab315879) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Rat kidney tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Rat cerebellum tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 4 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 160 kDa, 36 kDa
Western blot - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Low expression: brain, spleen (PMID: 2533512).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Factor H antibody [EPR26491-30] (Anti-Factor H antibody [EPR26491-30] ab315879) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 160 kDa, 124 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Mouse non-perfused fixed liver (B) Mouse perfused fixed liver tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/500 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) plasma of non-perfused fixed mouse liver. No staining on (B) perfused fixed mouse liver. (PMID: 32369457).

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Rat non-perfused fixed liver (B) Rat perfused fixed liver tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/500 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) plasma of non-perfused fixed rat liver. No staining on (B) perfused fixed rat liver.

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/500 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cerebrum.

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/500 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum.

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/200 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum.

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/200 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on plasma of human colorectal carcinoma.

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody [EPR26491-30] - BSA and Azide free (ab315880)
This data was developed using Anti-Factor H antibody [EPR26491-30] ab315879, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Factor H with Anti-Factor H antibody [EPR26491-30] ab315879 at 1/200 (0.976 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on plasma of human lung.

The section was incubated with Anti-Factor H antibody [EPR26491-30] ab315879 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins