Rabbit Recombinant Monoclonal Factor H antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--western-blot-img351.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539)")
![Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--western-blot-img50617.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539)")
![Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--western-blot-img116751.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539)")
![OI-RD Scanning - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--oi-rd-scanning-img98935.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed:21285368, PubMed:25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed:19503104, PubMed:26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed:18252712, PubMed:23332154, PubMed:28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed:20008295, PubMed:9558116). (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
Alternative names
HF, HF1, HF2, CFH, Complement factor H, H factor 1
Recommended products
Rabbit Recombinant Monoclonal Factor H antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6225
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 9.4 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248539 is the carrier-free version of Anti-Factor H antibody [EPR6225] ab133536.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
- Biological function summary
Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.
- Pathways
Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.
- Associated diseases and disorders
Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--western-blot-img351.jpg "Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)")
Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)
This data was developed using Anti-Factor H antibody [EPR6225] ab133536, the same antibody clone in a different buffer formulation.
Lanes 1-3: Merged signal (red and green). Green - Anti-Factor H antibody [EPR6225] ab133536 observed at 180 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-Factor H antibody [EPR6225] ab133536 Anti-Factor H antibody [EPR6225] was shown to specifically react with Factor H in wild-type A549 cells. Loss of signal was observed when knockout cell line Human CFH (Factor H) knockout A549 cell line ab267031 (knockout cell lysate Human CFH (Factor H) knockout A549 cell lysate ab257150) was used. Wild-type and Factor H knockout samples were subjected to SDS-PAGE. Anti-Factor H antibody [EPR6225] ab133536 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Factor H antibody [EPR6225] (Anti-Factor H antibody [EPR6225] ab133536) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: CFH knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human CFH (Factor H) knockout A549 cell line (Human CFH (Factor H) knockout A549 cell line ab267031)
Lane 3: HaCaT cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 180 kDa
![Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--western-blot-img50617.jpg "Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)")
Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)
This data was developed using Anti-Factor H antibody [EPR6225] ab133536, the same antibody clone in a different buffer formulation.
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with Anti-Factor H antibody [EPR6225] ab133536 overnight at 4°C at a 1/1000 dilution. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Factor H antibody [EPR6225] (Anti-Factor H antibody [EPR6225] ab133536) at 1/1000 dilution
Lane 1: Purified Factor H protein at 0.5 µg
Lane 2: Purified Factor H protein at 0.1 µg
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 170 kDa
![Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--western-blot-img116751.jpg "Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)")
Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)
This data was developed using Anti-Factor H antibody [EPR6225] ab133536, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Factor H antibody [EPR6225] (Anti-Factor H antibody [EPR6225] ab133536) at 1/1000 dilution
Lane 1: Human plasma lysate at 10 µg
Lane 2: Human kidney lysate at 10 µg
Lane 3: Human fetal lung lysate at 10 µg
Lane 4: Human fetal liver lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 139 kDa
Observed band size: 180 kDa
![OI-RD Scanning - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539), expandable thumbnail](https://content.abcam.com/products/images/factor-h-antibody-epr6225-bsa-and-azide-free-ab248539--oi-rd-scanning-img98935.jpg "OI-RD Scanning - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)")
OI-RD Scanning - Anti-Factor H antibody [EPR6225] - BSA and Azide free (ab248539)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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