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Mouse Monoclonal Factor H antibody. Suitable for ELISA, WB and reacts with Recombinant full length protein - Human, Human, Purified native protein samples. Cited in 8 publications.

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Images

Western blot - Anti-Factor H antibody [OX-24] (AB118820), expandable thumbnail
  • ELISA - Anti-Factor H antibody [OX-24] (AB118820), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Human
Expected
Tested
Purified native protein
Not recommended
Tested
Recombinant full length protein - Human
Tested
Not recommended

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
10 µg/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Purified native protein
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Purified native protein
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

1 product for Alternative Version

Target data

Function

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed:21285368, PubMed:25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed:19503104, PubMed:26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed:18252712, PubMed:23332154, PubMed:28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed:20008295, PubMed:9558116). (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.

Alternative names

HF, HF1, HF2, CFH, Complement factor H, H factor 1

Recommended products

Mouse Monoclonal Factor H antibody. Suitable for ELISA, WB and reacts with Recombinant full length protein - Human, Human, Purified native protein samples. Cited in 8 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
OX-24
Purification technique
Affinity purification Protein G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab118820 has switched from ascites to TCS on 19th September 2019. Lot numbers higher than GR3258447 are from tissue culture supernatant.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.

Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Associated diseases and disorders

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Factor H antibody [OX-24] (ab118820), expandable thumbnail

    Western blot - Anti-Factor H antibody [OX-24] (ab118820)

    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab118820 overnight at 4°C at a 1ug/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution for 1 hour at room temperature before imaging.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Factor H antibody [OX-24] (ab118820) at 1 µg/mL

    Lane 1: Human serum diluted 1/100 at 10 µL

    Lane 2: Human plasma diluted 1/100 at 10 µL

    Lane 3: Purified Factor H protein at 0.5 µg

    Lane 4: Purified Factor H protein at 0.1 µg

    Performed under reducing conditions.

    Predicted band size: 139 kDa

    Observed band size: 170 kDa

  • ELISA - Anti-Factor H antibody [OX-24] (ab118820), expandable thumbnail

    ELISA - Anti-Factor H antibody [OX-24] (ab118820)

    96-well microtitre plates were coated overnight at 4°C with recombinant human C3, C3b, iC3b, and Factor H proteins, in duplicate at a concentration of 1μg/mL. Plates were blocked with 1% BSA in PBS-T (0.1% Tween®) for 1 hour before incubation with a 10-step 4x serial dilution of ab118820 from 10μg/mL for 1 hour at room temperature. Antibody binding was detected with Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab6789) secondary antibody at a 1 in 10000 dilution for 1 hour at room temperature. Plates were incubated with TMB ELISA substrate for 7 minutes prior to being stopped with Stop solution and absorbance measured at 450nm.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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