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AB52244

Anti-Factor I/CFI antibody [3D6] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal Complement Factor I antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IF, CFI, Complement factor I, C3B/C4B inactivator

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D6

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab52244 is specific for the a-chain of human Factor I/CFI.

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Alternative Products

Primary Antibodies

AB231969

Anti-Factor I/CFI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor I/CFI antibody (AB231969)

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Complementary Products

Assay Kits

AB284615

Human Complement Factor I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Complement Factor I ELISA Kit (AB284615)

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Primary Antibodies

AB205198

Anti-AACT antibody [EPR17088-68]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] (AB205198)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Primary Antibodies

AB204917

Anti-Factor D/CFD antibody [EPR14699]

RabMAb|Recombinant

Western blot - Anti-Factor D/CFD antibody [EPR14699] (AB204917)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor I also known as Complement Factor I (CFI) is a serine protease involved in the complement system. Its molecular mass is approximately 88 kDa consisting of a heavy and a light chain linked by a disulfide bond. This protein is mainly expressed in the liver and circulates in the plasma. Factor I inhibits the complement pathways by cleaving complement components C3b and C4b preventing the formation of C3 and C5 convertases.
Biological function summary

Factor I plays a role in maintaining immune homeostasis by preventing excessive complement activation. It acts within a complex that includes cofactors such as Factor H Complement Factor 4 binding protein (C4BP) and Membrane Cofactor Protein (MCP). These interactions allow Factor I to selectively degrade activated complement components ensuring that complement activity is kept in check to avoid host cell damage.

Pathways

Factor I operates in both the classical and alternative complement pathways. In the classical pathway it regulates C3b and C4b breakdown to control the formation of the C3 convertase. In the alternative pathway Factor I works in conjunction with Factor H to degrade C3b. This regulation is important to prevent runaway activation protecting healthy tissue from complement-mediated damage.

Factor I deficiencies can lead to conditions like atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS insufficient Factor I activity permits unregulated complement activation leading to thrombotic microangiopathy. In AMD aberrant complement activation contributes to retinal damage and degeneration. These conditions illustrate the need for balanced Factor I activity to prevent disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed : 17320177, PubMed : 7360115). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed : 12055245, PubMed : 2141838, PubMed : 9605165). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed : 28671664).
See full target information CFI
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of aging 33:1507-21 PubMed21514693

2011

Ovarian hormone loss induces bioenergetic deficits and mitochondrial β-amyloid.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Yao,Ronald Irwin,Shuhua Chen,Ryan Hamilton,Enrique Cadenas,Roberta Diaz Brinton
View all publications
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Product promise

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