AB255824

Anti-Factor IX/PTC antibody [EPR22846-238]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Factor IX/PTC antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

2 Images

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Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

Factor IX/PTC was immunoprecipitated from 0.35 mg Human plasma with ab255824 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255824 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human plasma 10μg

Lane 2 : ab255824 IP in Human plasma

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255824 in Human plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824)

Predicted band size: 52 kDa

Observed band size: 45 kDa,66 kDa

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Lab

Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 6 seconds.

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST.

Factor IX is a glycoprotein. Zymogen FIX (66 kDa), intermediate FIXα (45 kDa), and heavy chain of FIXaβ (30 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20110423, 2294100, 18192270).

All lanes:

Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824) at 1/1000 dilution

Lane 1:

Human liver lysate at 20 µg

Lane 2:

Human plasma at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 30 kDa,45 kDa,66 kDa

false

Carrier free

Anti-Factor IX/PTC antibody [EPR22846-238] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22846-238

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.

Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).

See full target information Coagulation factor IX

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular genetics & genomic medicine 11:e2147 PubMed36795372

2023

Molecular pathogenesis of a novel Met394Thr variant causing hemophilia B.

Applications

Unspecified application

Species

Unspecified reactive species

Linna Lu,Lingyu Wang,Wukang Shen,Shuai Fang,Lidong Zhao,Xuchen Hu,Linhua Yang,Gang Wang

Cell death & disease 14:106 PubMed36774339

2023

DNMT3a-dermatopontin axis suppresses breast cancer malignancy via inactivating YAP.

Applications

Unspecified application

Species

Unspecified reactive species

Danrong Ye,Yuying Wang,Xiaochong Deng,Xiqian Zhou,Diya Liu,Baian Zhou,Wenfang Zheng,Xuehui Wang,Lin Fang

Stem cell research & therapy 13:353 PubMed35883203

2022

Generation of an mESC model with a human hemophilia B nonsense mutation via CRISPR/Cas9 technology.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Ma,Wenwen Sun,Lidong Zhao,Mingze Yao,Changxin Wu,Pengfei Su,Linhua Yang,Gang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Factor IX/PTC antibody [EPR22846-238]

Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise