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Rabbit Recombinant Monoclonal Factor IX/PTC antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824), expandable thumbnail
  • Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

Alternative names

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

Recommended products

Rabbit Recombinant Monoclonal Factor IX/PTC antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22846-238

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824), expandable thumbnail

    Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824)

    Factor IX/PTC was immunoprecipitated from 0.35 mg Human plasma with ab255824 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255824 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human plasma 10μg

    Lane 2: ab255824 IP in Human plasma

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab255824 in Human plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824)

    Predicted band size: 52 kDa

    Observed band size: 45 kDa, 66 kDa

  • Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824), expandable thumbnail

    Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824)

    Exposure time: Lane 1:3 minutes; Lane 2:6 seconds.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    Factor IX is a glycoprotein. Zymogen FIX (66 kDa), intermediate FIXα (45 kDa), and heavy chain of FIXaβ (30 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 20110423, 2294100, 18192270).

    All lanes: Western blot - Anti-Factor IX/PTC antibody [EPR22846-238] (ab255824) at 1/1000 dilution

    Lane 1: Human liver lysate at 20 µg

    Lane 2: Human plasma at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 52 kDa

    Observed band size: 30 kDa, 45 kDa, 66 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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