Anti-Factor IX/PTC antibody [EPR28007-19]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (AB318268)

Immunohistochemical analysis of paraffin-embedded Mouse perfused fixed skeletal muscle tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse perfused fixed skeletal muscle.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (AB318268)

Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed cardiac muscle. (B) Mouse non-perfused fixed cardiac muscle. tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nearly no staining in (A) mouse perfused fixed cardiac muscle; positive staining in plasma of (B) mouse non-perfused fixed cardiac muscle.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor IX/PTC antibody [EPR28007-19] (AB318268)

Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed liver. (B) Mouse non-perfused fixed liver. tissue labeling Factor IX/PTC with ab318268 at 1/100 (4.91 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in both (A) mouse perfused fixed liver and (B) mouse non-perfused fixed liver.
The section was incubated with ab318268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] (AB318268)

Negative control : brain, skeletal muscle(PMID : 2323576)

Lane4 was developed using a higher sensitivity ECL substrate.

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Factor IX/PTC antibody [EPR28007-19] (ab318268) at 1/1000 dilution

Lane 1:

Mouse plasma lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,37 kDa,36 kDa

false

Exposure time: 180s