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AB234849

Anti-Factor V antibody

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(2 Publications)

Rabbit Polyclonal Factor V antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human F5 aa 1450-1650.

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor V antibody (AB234849)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor V antibody (AB234849)

Paraffin-embedded human liver cancer tissue stained for Factor V with ab234849 (1/100 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Factor V antibody (AB234849)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Factor V antibody (AB234849)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Factor V using ab234849 at a dilution of 1/100 in ICC/IF. Secondary antibody used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human F5 aa 1450-1650. The exact immunogen used to generate this antibody is proprietary information.

P12259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 608:405-412 PubMed35922506

2022

Cellular recovery after prolonged warm ischaemia of the whole body.

Applications

Unspecified application

Species

Unspecified reactive species

David Andrijevic,Zvonimir Vrselja,Taras Lysyy,Shupei Zhang,Mario Skarica,Ana Spajic,David Dellal,Stephanie L Thorn,Robert B Duckrow,Shaojie Ma,Phan Q Duy,Atagun U Isiktas,Dan Liang,Mingfeng Li,Suel-Kee Kim,Stefano G Daniele,Khadija Banu,Sudhir Perincheri,Madhav C Menon,Anita Huttner,Kevin N Sheth,Kevin T Gobeske,Gregory T Tietjen,Hitten P Zaveri,Stephen R Latham,Albert J Sinusas,Nenad Sestan

iScience 25:103971 PubMed35224470

2022

Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Prasanti Kotagiri,Paul A Lyons,Rafia S Al-Lamki,Federica Mescia,Laura Bergamaschi,Lorinda Turner,Michael D Morgan,Fernando J Calero-Nieto,Karsten Bach,Nicole Mende,Nicola K Wilson,Emily R Watts,Patrick H Maxwell,Patrick F Chinnery,Nathalie Kingston,Sofia Papadia,Kathleen E Stirrups,Neil Walker,Ravindra K Gupta,David K Menon,Kieren Allinson,Sarah J Aitken,Mark Toshner,Michael P Weekes,James A Nathan,Sarah R Walmsley,Willem H Ouwehand,Mary Kasanicki,Berthold Göttgens,John C Marioni,Kenneth G C Smith,Jordan S Pober,John R Bradley
View all publications
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