JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB281679

Anti-Factor V antibody [EPR24284-60] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Factor V antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

1 Images
Sandwich ELISA - Anti-Factor V antibody [EPR24284-60] - BSA and Azide free (Capture) (AB281679)
  • sELISA

Lab

Sandwich ELISA - Anti-Factor V antibody [EPR24284-60] - BSA and Azide free (Capture) (AB281679)

Sandwich ELISA of ab253760 with the capture antibody ab281679 dilution at 2 µg/mL and detector antibody ab281678 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24284-60

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab281679 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Factor V. The recommended pair for sandwich ELISA is:
Capture: ab281679, Human Factor V Capture Antibody (unconjugated)
Detector: ab281678, Human Factor V Detector Antibody (unconjugated)
The reference range value is 15.63-2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB137976

Human Factor V ELISA Kit

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

  • 0
  • 0
Primary Antibodies

AB92621

Anti-Thrombin antibody

Western blot - Anti-Thrombin antibody (AB92621)

  • 4
  • 5
Primary Antibodies

AB196023

Anti-Factor X antibody [EPR16249]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor X antibody [EPR16249] (AB196023)

  • 0
  • 0
Primary Antibodies

AB289971

Anti-HC-II antibody [EPR25618-1]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] (AB289971)

  • 0
  • 0
Primary Antibodies

AB255824

Anti-Factor IX/PTC antibody [EPR22846-238]

RabMAb|Recombinant

Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com