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AB108614

Anti-Factor V antibody [EPR5191]

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(4 Publications)

Rabbit Recombinant Monoclonal Factor V antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

1 Images
Western blot - Anti-Factor V antibody [EPR5191] (AB108614)
  • WB

Unknown

Western blot - Anti-Factor V antibody [EPR5191] (AB108614)

All lanes:

Western blot - Anti-Factor V antibody [EPR5191] (ab108614) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human platelet lysate at 10 µg

Predicted band size: 252 kDa

false

  • Carrier free

    Anti-Factor V antibody [EPR5191] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5191

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB48230

Biotin Anti-Factor V antibody

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Primary Antibodies

AB234849

Anti-Factor V antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor V antibody (AB234849)

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Complementary Products

Assay Kits

AB137976

Human Factor V ELISA Kit

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

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Primary Antibodies

AB92621

Anti-Thrombin antibody

Western blot - Anti-Thrombin antibody (AB92621)

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Primary Antibodies

AB196023

Anti-Factor X antibody [EPR16249]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor X antibody [EPR16249] (AB196023)

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Primary Antibodies

AB289971

Anti-HC-II antibody [EPR25618-1]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] (AB289971)

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Primary Antibodies

AB255824

Anti-Factor IX/PTC antibody [EPR22846-238]

RabMAb|Recombinant

Immunoprecipitation - Anti-Factor IX/PTC antibody [EPR22846-238] (AB255824)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2404 PubMed38493152

2024

Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Watanabe,Yoshiaki Kise,Kento Yonezawa,Mariko Inoue,Nobutaka Shimizu,Osamu Nureki,Kenji Inaba

Transfusion 60:401-413 PubMed31785023

2019

Clinical manifestation of hemophilia A in the absence of mutations in the F8 gene that encodes FVIII: role of microRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna I Jankowska,Joseph McGill,Behnaz Pezeshkpoor,Johannes Oldenburg,Chintamani D Atreya,Zuben E Sauna

Nature communications 10:3224 PubMed31324782

2019

Active thrombin produced by the intestinal epithelium controls mucosal biofilms.

Applications

WB

Species

Human

Jean-Paul Motta,Alexandre Denadai-Souza,David Sagnat,Laura Guiraud,Anissa Edir,Chrystelle Bonnart,Mireille Sebbag,Perrine Rousset,Ariane Lapeyre,Carine Seguy,Noa Mathurine-Thomas,Heather J Galipeau,Delphine Bonnet,Laurent Alric,Andre G Buret,John L Wallace,Antoine Dufour,Elena F Verdu,Morley D Hollenberg,Eric Oswald,Matteo Serino,Celine Deraison,Nathalie Vergnolle

Cancer gene therapy 23:303-14 PubMed27492853

2016

Retargeted adenoviruses for radiation-guided gene delivery.

Applications

Flow Cyt

Species

Human

S A Kaliberov,L N Kaliberova,H Yan,V Kapoor,D E Hallahan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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