AB247698

Anti-Factor V antibody [EPR5191] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Factor V antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

1 Images

Unknown

Western blot - Anti-Factor V antibody [EPR5191] - BSA and Azide free (AB247698)

This data was developed using ab108614, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Factor V antibody [EPR5191] (<a href='/en-us/products/primary-antibodies/factor-v-antibody-epr5191-ab108614'>ab108614</a>) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human platelet lysate at 10 µg

Predicted band size: 252 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5191

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab247698 is the carrier-free version of ab108614.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.

Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

See full target information F5

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Factor V antibody [EPR5191] - BSA and Azide free

Western blot - Anti-Factor V antibody [EPR5191] - BSA and Azide free (AB247698)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise