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AB317221

Anti-Factor V antibody [HL2421]

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Rabbit Monoclonal Factor V antibody. Suitable for WB and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human F5.

View Alternative Names

Coagulation factor V, Activated protein C cofactor, F5

2 Images
Western blot - Anti-Factor V antibody [HL2421] (AB317221)
  • WB

Supplier Data

Western blot - Anti-Factor V antibody [HL2421] (AB317221)

Human plasma (30 ug) was separated by 5% SDS-PAGE and the membrane was blotted with ab317221 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Factor V antibody [HL2421] (ab317221) at 1/1000 dilution

All lanes:

Human plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Factor V antibody [HL2421] (AB317221)
  • WB

Supplier Data

Western blot - Anti-Factor V antibody [HL2421] (AB317221)

Rat plasma (50 ug) was separated by 5% SDS-PAGE and the membrane was blotted with ab317221 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Factor V antibody [HL2421] (ab317221) at 1/1000 dilution

All lanes:

Rat plasma at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2421

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human F5.

P12259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor V also known as proaccelerin is a coagulation factor playing a main role in blood clotting. This protein with a molecular mass of approximately 330 kDa is synthesized in the liver and found in plasma. The Factor V protein is essential for the coagulation cascade functioning as a pivotal mediator in the conversion of prothrombin to thrombin. It exists in plasma in an inactive form until activated by thrombin or factor Xa to fulfill its mechanical function in hemostasis.
Biological function summary

The mechanism of Factor V involves its function as a cofactor to Factor Xa in the prothrombinase complex. This complex formation enhances the efficiency of thrombin generation considerably accelerating clot formation. Factor V once converted to its active form aids in forming a stable clot by bridging and immobilizing cellular elements and fibrin strands. Expression occurs mainly in the liver but it also manifests in platelets where it enhances thrombin production during platelet activation and aggregation.

Pathways

The contribution of Factor V to coagulation is central within the hemostasis pathway specifically the intrinsic and extrinsic pathways of the coagulation cascade. Factor V binds with Factor X to form the prothrombinase complex which is important for the conversion of prothrombin to thrombin. Other proteins such as Factor VIII interact within this pathway to ensure blood clots form properly. Any disturbances in Factor V activity can significantly alter the normal flow of these pathways affecting thrombin generation and subsequent clotting.

Factor V mutations or deficiencies associate with conditions like Factor V Leiden and Factor V deficiency. Factor V Leiden is a genetic mutation that elevates the risk of thrombophilia leading to increased clot formation in veins. On the other hand a deficiency in Factor V can lead to Parahemophilia characterized by a tendency for excessive bleeding. In both conditions the abnormal activity of Factor V affects the interaction with proteins in the coagulation cascade especially impacting the prothrombinase complex and Factor X.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
See full target information F5
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Product promise

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