AB97614

Anti-Factor VII antibody

Name: Anti-Factor VII antibody (ab97614) | Abcam
Brand: Abcam Limited
SKU: AB97614
Price: 535 USD
Availability: InStock

(1 Review)

(10 Publications)

Rabbit Polyclonal Factor VII antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human F7 aa 200-450.

View Alternative Names

Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, F7

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VII antibody (AB97614)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VII antibody (AB97614)

Immunohistochemistry analysis of paraffin-embedded N human (gastric carcinoma) tissue, using ab97614 at 1/100 dilution.

Unknown

Western blot - Anti-Factor VII antibody (AB97614)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Factor VII antibody (ab97614) at 1/500 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 52 kDa

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Unknown

Western blot - Anti-Factor VII antibody (AB97614)

10% SDS PAGE

All lanes:

Western blot - Anti-Factor VII antibody (ab97614) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human F7 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

P08709

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VII also known as factor 7 or factor seven is a plasma glycoprotein with a molecular mass of approximately 50 kDa. It plays an important role in the blood coagulation process. Factor VII is mainly produced in the liver and requires vitamin K for its synthesis. In the inactive form it circulates in the plasma but upon activation to factor 7a it initiates the extrinsic pathway of blood coagulation. This activation is a critical step in hemostasis which helps to prevent excessive bleeding.

Biological function summary

Factor 7a indirectly activates further components in the blood coagulation cascade. Upon activation factor 7a becomes a part of the tissue factor (TF) complex which includes tissue factor expressed on subendothelial cells. The complex triggers the activation of factor IX and factor X propagating the coagulation cascade downstream. This series of activations amplifies the signal for clot formation highlighting factor VII's integral role in initiating coagulation.

Pathways

Factor VII is primarily involved in the extrinsic pathway of the coagulation cascade. This pathway is initiated when factor 7a forms a complex with tissue factor a protein that becomes exposed at injury sites. Factor VII is also indirectly linked to the common coagulation pathway through activation of factor X. The interaction between these pathways ensures efficient and rapid clot formation. Additionally factor IX also interacts with factor VII in the intricate network of coagulation processes that sustain hemostasis.

Deficiencies or dysfunctions in factor VII can lead to coagulopathies such as congenital factor VII deficiency which results in excessively prolonged bleeding. Conversely elevated factor VII activity can contribute to thromboembolic disorders like myocardial infarction. In these conditions factor VII often interacts with proteins like factor X and tissue factor highlighting their clinical significance in managing blood coagulation abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

See full target information F7

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:104547 PubMed35754738

2022

Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Hayashi,Ippei Shimizu,Yohko Yoshida,Ryutaro Ikegami,Masayoshi Suda,Goro Katsuumi,Shinya Fujiki,Kazuyuki Ozaki,Manabu Abe,Kenji Sakimura,Shujiro Okuda,Toshiya Hayano,Kazuhiro Nakamura,Kenneth Walsh,Naja Zenius Jespersen,Søren Nielsen,Camilla Scheele,Tohru Minamino

The journal of gene medicine 24:e3398 PubMed34786791

2021

A novel compound heterozygous variant linked to hematuria in a family with hereditary factor VII deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Nan Hu,Yu-Mian Gan,Yan-Ping Zhang,Dan-Dan Ruan,Yao-Bin Zhu,Xin-Fu Lin,Zhu-Ting Fang,Li-Sheng Liao,Fa-Qiang Tang,Jie-Wei Luo

Vascular pharmacology 130:106679 PubMed32387621

2020

Effects of rivaroxaban and dabigatran on local expression of coagulation and inflammatory factors within human aortic stenotic valves.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Wypasek,Joanna Natorska,Piotr Mazur,Magdalena Kopytek,Bogusław Gawęda,Przemysław Kapusta,Jacek Madeja,Teresa Iwaniec,Bogusław Kapelak,Anetta Undas

Experimental and therapeutic medicine 17:2945-2952 PubMed30936964

2019

miR-204-5p promotes diabetic retinopathy development via downregulation of microtubule-associated protein 1 light chain 3.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Bang Mao,Yan-Hua Cheng,Yan-Ying Xu

Iranian journal of biotechnology 16:e1813 PubMed30555841

2018

Functional and Molecular Characterization of C91S Mutation in the Second Epidermal Growth Factor-Like Domain of Factor VII.

Applications

Unspecified application

Species

Unspecified reactive species

Amir Mashayekhi,Shirin Shahbazi,Mirdavood Omrani

Molecular medicine reports 17:2738-2742 PubMed29207137

2017

In vitro expression of mutant factor VII proteins and characterization of their clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Amir Mashayekhi,Shirin Shahbazi,Mirdavood Omrani,Reza Mahdian

Molecular therapy. Nucleic acids 9:274-283 PubMed29246306

2017

Factor VII-Induced MicroRNA-135a Inhibits Autophagy and Is Associated with Poor Prognosis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kuang-Tzu Huang,I-Ying Kuo,Ming-Chao Tsai,Chun-Hsien Wu,Li-Wen Hsu,Li-Yu Chen,Chao-Pin Kung,Yu-Fan Cheng,Shigeru Goto,Yu-Wei Chou,Chao-Long Chen,Chih-Che Lin,Kuang-Den Chen

Anticancer research 37:465-473 PubMed28179291

2017

Synergistic Inhibitory Effect of Traditional Chinese Medicine Astragaloside IV and Curcumin on Tumor Growth and Angiogenesis in an Orthotopic Nude-Mouse Model of Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Zhang,Decai Tang,Wenhua Zang,Gang Yin,Jianguo Dai,Y U Sun,Zhijian Yang,Robert M Hoffman,Xiuxia Guo

Cytotechnology 69:317-327 PubMed28070807

2017

Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kotomi Nagahashi,Kazuo Umemura,Naohiro Kanayama,Takayuki Iwaki

Cell death & disease 5:e1244 PubMed24853422

2014

Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma.

Applications

Species

Human

K-D Chen,C-C Wang,M-C Tsai,C-H Wu,H-J Yang,L-Y Chen,T Nakano,S Goto,K-T Huang,T-H Hu,C-L Chen,C-C Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Factor VII antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VII antibody (AB97614)

Western blot - Anti-Factor VII antibody (AB97614)

Western blot - Anti-Factor VII antibody (AB97614)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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