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AB259698

Anti-Factor VII antibody [EPR22364-119] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Factor VII antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, F7

1 Images
Sandwich ELISA - Anti-Factor VII antibody [EPR22364-119] - BSA and Azide free (Detector) (AB259698)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Factor VII antibody [EPR22364-119] - BSA and Azide free (Detector) (AB259698)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab190810).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22364-119

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab259698 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Factor VII. The recommended pair for sandwich ELISA is:
Capture: ab259697, Human Factor VII Capture Antibody (unconjugated)
Detector: ab259698, Human Factor VII Detector Antibody (unconjugated)
The reference range value is 0.39 - 25 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VII also known as factor 7 or factor seven is a plasma glycoprotein with a molecular mass of approximately 50 kDa. It plays an important role in the blood coagulation process. Factor VII is mainly produced in the liver and requires vitamin K for its synthesis. In the inactive form it circulates in the plasma but upon activation to factor 7a it initiates the extrinsic pathway of blood coagulation. This activation is a critical step in hemostasis which helps to prevent excessive bleeding.
Biological function summary

Factor 7a indirectly activates further components in the blood coagulation cascade. Upon activation factor 7a becomes a part of the tissue factor (TF) complex which includes tissue factor expressed on subendothelial cells. The complex triggers the activation of factor IX and factor X propagating the coagulation cascade downstream. This series of activations amplifies the signal for clot formation highlighting factor VII's integral role in initiating coagulation.

Pathways

Factor VII is primarily involved in the extrinsic pathway of the coagulation cascade. This pathway is initiated when factor 7a forms a complex with tissue factor a protein that becomes exposed at injury sites. Factor VII is also indirectly linked to the common coagulation pathway through activation of factor X. The interaction between these pathways ensures efficient and rapid clot formation. Additionally factor IX also interacts with factor VII in the intricate network of coagulation processes that sustain hemostasis.

Deficiencies or dysfunctions in factor VII can lead to coagulopathies such as congenital factor VII deficiency which results in excessively prolonged bleeding. Conversely elevated factor VII activity can contribute to thromboembolic disorders like myocardial infarction. In these conditions factor VII often interacts with proteins like factor X and tissue factor highlighting their clinical significance in managing blood coagulation abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
See full target information F7
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Product promise

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For full details, please see our Terms & Conditions

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