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AB171825

Anti-Factor VIII antibody

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(4 Publications)

Mouse Polyclonal Factor VIII antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human F8.

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

1 Images
Western blot - Anti-Factor VIII antibody (AB171825)
  • WB

Supplier Data

Western blot - Anti-Factor VIII antibody (AB171825)

All lanes:

Western blot - Anti-Factor VIII antibody (ab171825) at 1 µg/mL

Lane 1:

Factor VIII (isoform 2)-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 267 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human F8.

P00451

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
See full target information F8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of thrombosis and thrombolysis 57:1349-1362 PubMed39222205

2024

RNAi targeting LMAN1-MCFD2 complex promotes anticoagulation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Siqian Ma,Boyan Liu,Hong Du,Fei Yang,Jingjing Han,Xinqi Huang,Minyang Zhang,Shundong Ji,Miao Jiang

Journal of functional biomaterials 13: PubMed35645267

2022

Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Laercio Uemura,Rossana Baggio Simeoni,Paulo André Bispo Machado Júnior,Gustavo Gavazzoni Blume,Luize Kremer Gamba,Murilo Sgarbossa Tonial,Paulo Ricardo Baggio Simeoni,Victoria Stadler Tasca Ribeiro,Rodrigo Silvestre,Katherine Athayde Teixeira de Carvalho,Marcelo Henrique Napimoga,Júlio Cesar Francisco,Luiz Cesar Guarita-Souza

Reproductive biology and endocrinology : RB&E 19:187 PubMed34906165

2021

Increased METTL3-mediated mA methylation inhibits embryo implantation by repressing HOXA10 expression in recurrent implantation failure.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Xue,Wenbo Zhou,Wenqiang Fan,Jianya Jiang,Chengcai Kong,Wei Zhou,Jianmei Zhou,Xiaoyang Huang,Haiyan Yang,Qian Han,Bin Zhang,Lingyun Xu,Bin Yu,Li Chen

Mediterranean journal of hematology and infectious diseases 13:e2021041 PubMed34276910

2021

Hsa-miR-5581-3p and Hsa-miR-542-3p Target the Gene in Hemophilia A without Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Feiying Meng
View all publications
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