AB203590

Anti-Factor VIII antibody

Name: Anti-Factor VIII antibody (ab203590) | Abcam
Brand: Abcam Limited
SKU: AB203590
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Factor VIII antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human F8 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB203590)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB203590)

Immunohistochemical analysis of formalin-fixed, paraffin embedded Human skin tissue labeling Factor VIII with ab203590 at a 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human F8 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P00451

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe please use a dilution of 1/50 - 1/200.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.

Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

See full target information F8

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Burns & trauma 10:tkac035 PubMed35937591

2022

Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction .

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Yuan,Xianlan Duan,Zhao Li,Bin Yao, Enhejirigala,Wei Song,Yi Kong,Yuzhen Wang,Fanliang Zhang,Liting Liang,Shijun Zhu,Mengde Zhang,Chao Zhang,Sha Huang,Xiaobing Fu

Experimental and therapeutic medicine 14:3632-3636 PubMed29042958

2017

Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Deng,Na Lin,Leiyun Wu,Qaing Jia,Hua Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Factor VIII antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB203590)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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