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AB236284

Anti-Factor VIII antibody

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(8 Publications )

Rabbit Polyclonal Factor VIII antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human F8.

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Factor VIII antibody (AB236284)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Factor VIII antibody (AB236284)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Factor VIII using ab236284 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB236284)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB236284)

Paraffin-embedded human placental tissue stained for Factor VIII using ab236284 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB236284)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody (AB236284)

Paraffin-embedded human renal tissue stained for Factor VIII using ab236284 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human F8.

P00451

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [EPR12011] (AB174290)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
See full target information F8
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1114321 PubMed36911720

2023

Head and neck dermatitis is exacerbated by colonization, skin barrier disruption, and immune dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Howard Chu,Su Min Kim,KeLun Zhang,Zhexue Wu,Hemin Lee,Ji Hye Kim,Hye Li Kim,Yu Ri Kim,Seo Hyeong Kim,Wan Jin Kim,Yang Won Lee,Kwang Hoon Lee,Kwang-Hyeon Liu,Chang Ook Park

Respiratory research 23:100 PubMed35459189

2022

PEDF is an antifibrosis factor that inhibits the activation of fibroblasts in a bleomycin-induced pulmonary fibrosis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Xichun Qin,Caili Jia,Jingtian Liang,Jiali Chen,Xiucheng Liu,Zhixiang Chao,Hao Qin,Yanliang Yuan,Zhiwei Liu,Zhongming Zhang,Hongyan Dong,Hao Zhang

Nature communications 13:422 PubMed35058465

2022

Correction of a Factor VIII genomic inversion with designer-recombinases.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Lansing,Liliya Mukhametzyanova,Teresa Rojo-Romanos,Kentaro Iwasawa,Masaki Kimura,Maciej Paszkowski-Rogacz,Janet Karpinski,Tobias Grass,Jan Sonntag,Paul Martin Schneider,Ceren Günes,Jenna Hoersten,Lukas Theo Schmitt,Natalia Rodriguez-Muela,Ralf Knöfler,Takanori Takebe,Frank Buchholz

Frontiers in cell and developmental biology 9:672159 PubMed34336829

2021

Comparative Transcriptional Analysis of Pulmonary Arterial Hypertension Associated With Three Different Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Zhenhong Jiang,Dandan Zhang,Linghua Fu,Rong Wan,Kui Hong

IUBMB life 73:927-940 PubMed33890394

2021

miR-543 in human mesenchymal stem cell-derived exosomes promotes cardiac microvascular endothelial cell angiogenesis after myocardial infarction through COL4A1.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Yang,Xueting Liu,Manli Jiang,Jian Li,Yaping Tang,Lin Zhou

International journal of molecular medicine 46:1671-1682 PubMed33000267

2020

Insulin‑receptor substrate 1 protects against injury in endothelial cell models of ox‑LDL‑induced atherosclerosis by inhibiting ER stress/oxidative stress‑mediated apoptosis and activating the Akt/FoxO1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Liu,Xu Yi,Yuan Tao,Yanjiang Wang,Zhiqiang Xu

Bioscience reports 39: PubMed31383785

2019

polysaccharide induced apoptosis and inhibited proliferation in infantile hemangioma endothelial cells via down-regulation of PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Lou,Guo Chen,Bing Zhong,Feng Liu

Oncology letters 16:6988-6997 PubMed30546432

2018

Clinical diagnosis of adult patients with acute megakaryocytic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Guangjie Zhao,Wanling Wu,Xiaoqin Wang,Jingwen Gu
View all publications
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Product promise

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