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AB78852

Anti-Factor VIII antibody [GMA-012]

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(4 Publications)

Mouse Monoclonal Factor VIII antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human F8.

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

2 Images
Western blot - Anti-Factor VIII antibody [GMA-012] (AB78852)
  • WB

Unknown

Western blot - Anti-Factor VIII antibody [GMA-012] (AB78852)

All lanes:

Western blot - Anti-Factor VIII antibody [GMA-012] (ab78852) at 1 µg/mL

All lanes:

human Factor VIII at 2 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugated IgG

Predicted band size: 267 kDa

Observed band size: 280 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [GMA-012] (AB78852)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [GMA-012] (AB78852)

IHC image of Factor VIII staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78852, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GMA-012

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human F8.

P00451

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography DEAE-C
Storage buffer
pH: 7.4 Constituents: 1% Mannitol, 0.87% Sodium chloride, 0.164% Sodium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
See full target information F8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 55:1296-1312 PubMed31638182

2019

Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiping Ye,Zhentian Ni,Shen Yicheng,Hao Pan,Yongyi Huang,Ying Xiong,Te Liu

Experimental and therapeutic medicine 18:3913-3919 PubMed31641378

2019

Homocysteine inhibits the viability and migration ability of human umbilical vein endothelial cells by downregulating the expression of vascular endothelial growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yu,Ling-Ling Zhang,Xiao-Peng Wu,Rui Zhao,Zhi-Xin Meng,Kun Wang,Bin Wang,Hui Wang,Zuo-Lei Shi,Guang-Xin Li

Experimental and therapeutic medicine 18:1752-1760 PubMed31410134

2019

Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low-density lipoprotein-induced human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Gong,Congyang Liu,Hongling Wang,Jinying Fan,Cuihong Jiang,Yong Zou

Journal of neurochemistry 139:757-768 PubMed27565895

2016

β-Caryophyllene protects in vitro neurovascular unit against oxygen-glucose deprivation and re-oxygenation-induced injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocui Tian,Jianhua Peng,Jianjun Zhong,Mei Yang,Jinwei Pang,Jie Lou,Minghang Li,Ruidi An,Qian Zhang,Lu Xu,Zhi Dong
View all publications
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