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AB168752

Anti-Factor VIII + VIII C2domain antibody

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(1 Publication)

Rabbit Polyclonal Factor VIII antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human F8.

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

1 Images
Western blot - Anti-Factor VIII + VIII C2domain antibody (AB168752)
  • WB

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Western blot - Anti-Factor VIII + VIII C2domain antibody (AB168752)

All lanes:

Western blot - Anti-Factor VIII + VIII C2domain antibody (ab168752) at 1 µg/mL

Lane 1:

Factor VIII + VIII A2domain transfected 293T cell lysate (24.60 KDa) at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated secondary antibody at 1/7500 dilution

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human F8.

P00451

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII often called antihemophilic factor is a glycoprotein essential for blood coagulation. It exists with its C2 domain important for its interaction with other molecules. Factor VIII's mass is approximately 265 kDa. The liver mainly produces Factor VIII and it circulates in plasma as a complex with von Willebrand factor (vWF) helping maintain its stability.
Biological function summary

Factor VIII participates in the coagulation cascade where it acts as a cofactor to Factor IXa. This complex activates Factor X which then converts prothrombin to thrombin facilitating clot formation. The C2 domain of Factor VIII helps in binding to phospholipid surfaces and vWF ensuring proper localization during hemostasis. Factor VIII is therefore an important part of the blood clotting complex.

Pathways

Factor VIII operates centrally in the intrinsic pathway of the hemostasis process. Within this pathway it collaborates closely with Factor IX and plays a part in the buildup to thrombin generation. Another key pathway involving Factor VIII is the common coagulation pathway which eventually leads to fibrin clot formation. Related proteins include Factor IX and Factor X both essential for the proper functioning of the coagulation mechanism.

Deficiencies or dysfunctions in Factor VIII lead to hemophilia A a genetic blood-clotting disorder marked by prolonged bleeding. This disorder results from mutations leading to a deficiency of functional Factor VIII. Von Willebrand disease also involves Factor VIII as vWF stabilizes and protects it in circulation. Patients with von Willebrand disease often have reduced levels of Factor VIII or impaired function due to the destabilization in the absence of vWF.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
See full target information F8

Additional targets

F8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 12:5100-5110 PubMed39055233

2024

Restoring energy metabolism by NAD supplement prevents alcohol-induced liver injury and boosts liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Liu,Cheng Cheng,Han Gao,Xue-Jin Zhu,Xian He,Ming-Xi Zhou,Yuan Gao,Ya-Wen Lu,Xin-Hua Song,Xiao-He Xiao,Jia-Bo Wang,Chun-Jun Xu,Zhi-Tao Ma
View all publications
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