AB79929

Anti-Factor X antibody

Name: Anti-Factor X antibody (ab79929) | Abcam
Brand: Abcam Limited
SKU: AB79929
Price: 535 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal Factor X antibody. Suitable for ELISA, Flow Cyt, WB, IHC-P, RIA, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human F10.

View Alternative Names

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

1 Images

ELISA - Anti-Factor X antibody (AB79929)

ELISA

Supplier Data

ELISA - Anti-Factor X antibody (AB79929)

Human Factor X Antibody Assay Performance. Assay was performed using increasing concentrations of biotinylated purified Human Factor X Protein. The antibody sensitivity is at 1 pg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

RIA, ELISA, Flow Cyt, WB, IHC-P, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human F10.

P00742

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.

Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).

See full target information F10

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37239814

2023

Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,James M Williams,Daniel T Lammers,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn

JCI insight 8: PubMed36802340

2023

Neutrophil extracellular traps contribute to coagulopathy after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Jin,Fang Wang,Jiawei Tian,Xinyi Zhao,Jiawei Dong,Nan Wang,Zhihui Liu,Hongtao Zhao,Wenqiang Li,Ge Mang,Shaoshan Hu

Vascular pharmacology 130:106679 PubMed32387621

2020

Effects of rivaroxaban and dabigatran on local expression of coagulation and inflammatory factors within human aortic stenotic valves.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Wypasek,Joanna Natorska,Piotr Mazur,Magdalena Kopytek,Bogusław Gawęda,Przemysław Kapusta,Jacek Madeja,Teresa Iwaniec,Bogusław Kapelak,Anetta Undas

EBioMedicine 53:102671 PubMed32114386

2020

Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhou,Tao Li,Jiaqi Jin,Yingmiao Liu,Baorong Li,Quanye Sun,Jiawei Tian,Hongtao Zhao,Zhihui Liu,Shuai Ma,Shuoqi Zhang,Valerie A Novakovic,Jialan Shi,Shaoshan Hu

Nature communications 10:3224 PubMed31324782

2019

Active thrombin produced by the intestinal epithelium controls mucosal biofilms.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Paul Motta,Alexandre Denadai-Souza,David Sagnat,Laura Guiraud,Anissa Edir,Chrystelle Bonnart,Mireille Sebbag,Perrine Rousset,Ariane Lapeyre,Carine Seguy,Noa Mathurine-Thomas,Heather J Galipeau,Delphine Bonnet,Laurent Alric,Andre G Buret,John L Wallace,Antoine Dufour,Elena F Verdu,Morley D Hollenberg,Eric Oswald,Matteo Serino,Celine Deraison,Nathalie Vergnolle

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Factor X antibody

ELISA - Anti-Factor X antibody (AB79929)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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