AB97632

Anti-Factor X antibody

Name: Anti-Factor X antibody (ab97632) | Abcam
Brand: Abcam Limited
SKU: AB97632
Price: 480 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Factor X antibody. Suitable for WB and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human F10 aa 1-300.

View Alternative Names

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

3 Images

Supplier Data

Western blot - Anti-Factor X antibody (AB97632)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Factor X antibody (ab97632) at 1/1000 dilution

All lanes:

HepG2 conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-Factor X antibody (AB97632)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Factor X antibody (ab97632) at 1/500 dilution

All lanes:

Rat liver tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-Factor X antibody (AB97632)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Factor X antibody (ab97632) at 1/5000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

Human F10-transfected 293T cell lysates at 30 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Human F10 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P00742

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.

Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427, PubMed : 39880037). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).

See full target information F10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684386

2024

Knockdown Induces Obesity and AHO Features in Early Zebrafish Larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa Abbas,Ayat S Hammad,Zain Z Zakaria,Maha Al-Asmakh,Khalid Hussain,Mashael Al-Shafai

EMBO reports 25:4950-4978 PubMed39358551

2024

SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular function.

Applications

Unspecified application

Species

Unspecified reactive species

Gyan Ranjan,Paras Sehgal,Vinod Scaria,Sridhar Sivasubbu

View all publications

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Product promise

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Anti-Factor X antibody

Western blot - Anti-Factor X antibody (AB97632)

Western blot - Anti-Factor X antibody (AB97632)

Western blot - Anti-Factor X antibody (AB97632)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise