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AB111171

Anti-Factor Xa antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Factor X antibody. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human F10.

View Alternative Names

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

1 Images
ELISA - Anti-Factor Xa antibody (AB111171)
  • ELISA

Supplier Data

ELISA - Anti-Factor Xa antibody (AB111171)

Human Factor Xa Antibody Assay Performance. Assay was performed using increasing concentrations of biotinylated Purified Human Factor Xa Protein. The antibody sensitivity is at 1 pg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human F10.

P00742

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor Xa also known as activated factor X is an important serine protease in the coagulation cascade. With a molecular mass around 37 kDa this enzyme is an important component in blood coagulation. Factor Xa derives from the hepatic synthesis of factor X requiring vitamin K for its gamma-carboxylation and it is found circulating within the blood plasma. The conversion of factor X into factor Xa occurs when exposed to either extrinsic or intrinsic coagulation pathways.
Biological function summary

Activated factor X plays a pivotal role in converting prothrombin into thrombin facilitating the cross-linking of fibrin which stabilizes the forming clot. Factor Xa while acting as a monomer often associates with factor Va to form prothrombinase complex enhancing its efficiency. The prothrombinase complex accelerates thrombin generation exponentially which is an essential step in hemostasis.

Pathways

The blood coagulation cascade involves factor Xa prominently within the intrinsic and extrinsic pathways. Factor Xa lies downstream in these pathways and links them to the common pathway. It directly interacts with thrombin and factor Va forming an integral part of both pathways in the conversion of prothrombin to thrombin. These steps are important in maintaining hemostasis and enabling proper clot formation during vascular injury.

Factor Xa is highly relevant in coagulopathies such as deep vein thrombosis (DVT) and pulmonary embolism (PE). Aberrant activity of this factor contributes to excessive clot formation leading to thrombotic disorders. Additionally factor Xa is a target for anticoagulant therapies such as rivaroxaban and apixaban which inhibit its activity to prevent thrombotic events. Such inhibitors affect conditions related to factor Xa impacting the risk and management of cardiovascular diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).
See full target information F10
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Vascular pharmacology 130:106679 PubMed32387621

2020

Effects of rivaroxaban and dabigatran on local expression of coagulation and inflammatory factors within human aortic stenotic valves.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Wypasek,Joanna Natorska,Piotr Mazur,Magdalena Kopytek,Bogusław Gawęda,Przemysław Kapusta,Jacek Madeja,Teresa Iwaniec,Bogusław Kapelak,Anetta Undas

Malaria journal 19:155 PubMed32295584

2020

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Tougan,Jyotheeswara R Edula,Masayuki Morita,Eizo Takashima,Hajime Honma,Takafumi Tsuboi,Toshihiro Horii

American journal of physiology. Lung cellular and 309:L449-62 PubMed26092993

2015

15-Lipoxygenase and 15-hydroxyeicosatetraenoic acid regulate intravascular thrombosis in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Shen,Jiucheng Shi,Na Wang,Xiufeng Yu,Chen Zhang,Jing Li,Liuping Wei,Cui Ma,Xijuan Zhao,Mingming Lian,Chun Jiang,Daling Zhu
View all publications
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