Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (AB242152)

Factor XII was immunoprecipitated from 0.35 mg of human plasma with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human plasma 10 μg (Input).

Lane 2 : ab242123 IP in human plasma.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242123 in human plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID : 8049433, PMID 2473984).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242123).

All lanes:

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (<a href='/en-us/products/primary-antibodies/factor-xii-antibody-epr22292-127-ab242123'>ab242123</a>)

Predicted band size: 67 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (AB242152)

Factor XII was immunoprecipitated from 0.35 mg of human serum with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human serum 10 μg (Input).

Lane 2 : ab242123 IP in human serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242123 in human serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID : 8049433, PMID 2473984).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242123).

All lanes:

Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (<a href='/en-us/products/primary-antibodies/factor-xii-antibody-epr22292-127-ab242123'>ab242123</a>)

Predicted band size: 25 kDa,67 kDa

false

• WB

Lab

Western blot - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (AB242152)

This data was developed using ab242123, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Anti-6X His tag® antibody [AD1.1.10] loading control staining at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human light chain Factor XII.

All lanes:

Western blot - Anti-Factor XII antibody [EPR22292-127] (<a href='/en-us/products/primary-antibodies/factor-xii-antibody-epr22292-127-ab242123'>ab242123</a>) at 1/1000 dilution

Lane 1:

His-tagged heavy chain of human Factor XII recombinant protein at 10 ng

Lane 2:

Lif/His-tagged light chain of human Factor XII recombinant protein at 10 ng

Lane 3:

No-tagged human Factor XII recombinant protein without His tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 65 kDa,50-70 kDa

false

Exposure time: 92s