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AB250814

Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Factor XIII antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Coagulation factor XIII B chain, Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain, F13B

3 Images
Immunoprecipitation - Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free (AB250814)
  • IP

Supplier Data

Immunoprecipitation - Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free (AB250814)

This data was developed using ab185215, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human plasma lysate immunoprecipitated using ab185215 at 1/40 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Factor XIII antibody [EPR10986(2)] (<a href='/en-us/products/primary-antibodies/factor-xiii-antibody-epr109862-ab185215'>ab185215</a>)

Predicted band size: 76 kDa

false

Western blot - Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free (AB250814)
  • WB

Supplier Data

Western blot - Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free (AB250814)

This data was developed using ab185215, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Factor XIII antibody [EPR10986(2)] (<a href='/en-us/products/primary-antibodies/factor-xiii-antibody-epr109862-ab185215'>ab185215</a>) at 1/10000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human placenta lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 76 kDa

false

Western blot - Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free (AB250814)
  • WB

Supplier Data

Western blot - Anti-Factor XIII antibody [EPR10986(2)] - BSA and Azide free (AB250814)

This data was developed using ab185215, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Factor XIII antibody [EPR10986(2)] (<a href='/en-us/products/primary-antibodies/factor-xiii-antibody-epr109862-ab185215'>ab185215</a>) at 1/50000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10986(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250814 is the carrier-free version of ab185215.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XIII also known as fibrin-stabilizing factor is an enzyme that plays a role in blood coagulation. It is a zymogen of approximately 320 kDa expressed primarily in the liver megakaryocytes and placenta. In its active form Factor XIII is a heterotetramer composed of two A subunits and two B subunits. The A subunits are the catalytic parts while the B subunits function as carriers. Factor XIII is widely present in plasma and in platelets aiding in clot stabilization.
Biological function summary

Factor XIII acts to strengthen and stabilize the fibrin clot by cross-linking fibrin molecules. The enzyme is part of a larger complex known as the blood coagulation pathway. Factor XIII becomes activated by thrombin in the presence of calcium ions. Upon activation it catalyzes the formation of covalent bonds between fibrin molecules enhancing the mechanical strength and resistance of the blood clot. This action prevents premature clot lysis and supports wound healing.

Pathways

Factor XIII plays a significant role in the coagulation cascade and fibrinolytic system. In the coagulation pathway it functions downstream of fibrin formation providing cross-linking important to clot integrity. Factor XIII is closely associated with proteins such as fibrinogen and thrombin which are key components in the clotting process. Additionally Factor XIII interacts with the fibrinolytic system connecting to proteins like plasmin that are essential for clot breakdown.

Malfunction or deficiency of Factor XIII can lead to bleeding disorders such as Factor XIII deficiency characterized by a tendency to bleed excessively. This deficiency severely impacts the stabilization of clots resulting in poor wound healing. Additionally abnormal Factor XIII activity can contribute to thrombotic disorders where excessive clot stabilization occurs linking Factor XIII to proteins involved in thrombus formation like fibrin and plasminogen. Understanding these interactions is key in managing related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin.
See full target information F13B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com