AB76105

Anti-Factor XIIIa antibody [EP3372]

Name: Anti-Factor XIIIa antibody [EP3372] (ab76105) | Abcam
Brand: Abcam Limited
SKU: AB76105
Price: 540 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal Factor XIIIa antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

F13A, F13A1, Coagulation factor XIII A chain, Coagulation factor XIIIa, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

ab76105, at 1/100 dilution, staining Factor XIIIa in paraffin-embedded human skin tissue by Immunohistochemistry. Note positive staining of dendrocytes within the papillary dermis.

Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Frozn sections) analysis of mouse spleen tissue sections labeling Factor XIIIa with purified ab76105 at 1 : 50 (1.3μg/ml). Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^© 488, ab150077) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. DAPI was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Factor XIIIa with purified ab76105 at 1 : 1000 (0.27 μg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse placenta tissue sections labeling Factor XIIIa with purified ab76105 at 1 : 1000 (0.27 μg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Frozn sections) analysis of mouse colon tissue sections labeling Factor XIIIa with purified ab76105 at 1 : 50 (1.3μg/ml). Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^© 488, ab150077) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. DAPI was used as a counterstain.

Unknown

Western blot - Anti-Factor XIIIa antibody [EP3372] (AB76105)

All lanes:

Western blot - Anti-Factor XIIIa antibody [EP3372] (ab76105) at 1/100000 dilution

All lanes:

PBMC cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

Unknown

Western blot - Anti-Factor XIIIa antibody [EP3372] (AB76105)

All lanes:

Western blot - Anti-Factor XIIIa antibody [EP3372] (ab76105) at 0.065 µg/mL

Lane 1:

Mouse plasma at 20 µg

Lane 2:

Mouse platelet at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 83 kDa

Observed band size: 80 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Factor XIIIa antibody [EP3372]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Factor XIIIa antibody [EP3372]
Carrier free

Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free
578 PE

PE Anti-Factor XIIIa antibody [EP3372]
660 APC

APC Anti-Factor XIIIa antibody [EP3372]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Factor XIIIa antibody [EP3372]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Factor XIIIa antibody [EP3372]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Factor XIIIa antibody [EP3372]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3372

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XIIIa also known as Factor 13a Factor XIII or Factor 13 is an enzyme that plays an important role in blood coagulation. Its mass is approximately 83 kDa. This enzyme is part of the transglutaminase family and is predominantly found in plasma and platelets. Factor XIIIa becomes active when thrombin and calcium ions cleave its A subunits converting it from an inactive zymogen known simply as Factor XIII or Factor 13 to its active form. The best assays for Factor XIIIa often measure its transglutaminase activity which facilitates the cross-linking of fibrin stabilizing blood clots.

Biological function summary

Factor XIIIa is important for hemostasis facilitating the final stages of blood clot formation by cross-linking fibrin polymers. This enzyme does not function in isolation; it forms a tetrameric complex with two A subunits and two B subunits. The active form Factor XIIIa catalyzes the formation of covalent bonds between glutamine and lysine residues in fibrin chains. This enhances the mechanical strength and resistance of the clot preventing premature degradation.

Pathways

Factor XIIIa is part of the coagulation pathway specifically involved in the stabilization of fibrin in the final steps of the cascade. It interacts closely with proteins like fibrinogen and thrombin as these proteins are essential in the conversion of Factor XIII to Factor XIIIa and in clot formation. Factor XIIIa also plays a role in the wound healing process by stabilizing the ECM (extracellular matrix) through cross-linking fibrin fibronectin and collagen.

Factor XIIIa deficiency can lead to bleeding diathesis a condition characterized by poor clot stability and prolonged bleeding. This deficiency is often congenital and involves reduced levels or activity of Factor XIII. Conversely overactivity or dysregulation of Factor XIIIa may contribute to thrombotic disorders where abnormal clot formation can lead to conditions like deep vein thrombosis. Clinically understanding Factor XIIIa interactions especially its functional relationships with fibrinogen and thrombin is critical in managing these bleeding and thrombotic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

See full target information F13A1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e70091 PubMed39601163

2024

Single-cell dissection reveals immunosuppressive F13A1+ macrophage as a hallmark for multiple primary lung cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglin Yang,Jiahao Qu,Jingting Wu,Songhua Cai,Wenyi Liu,Youjun Deng,Yiran Meng,Liuqing Zheng,Lishen Zhang,Li Wang,Xiaotong Guo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401095 PubMed38946578

2024

Genetically Engineered Membrane-Coated Nanoparticles for Enhanced Prostate-Specific Membrane Antigen Targeting and Ferroptosis Treatment of Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Li,Hongji Li,Keying Zhang,Chao Xu,Jingwei Wang,Zeyu Li,Yike Zhou,Shaojie Liu,Xiaolong Zhao,Zhengxuan Li,Fa Yang,Wei Hu,Yuming Jing,Peng Wu,Jingliang Zhang,Changhong Shi,Rui Zhang,Wenkai Jiang,Nianzeng Xing,Weihong Wen,Donghui Han,Weijun Qin

Nature genetics 56:442-457 PubMed38361033

2024

Multi-omic profiling of clear cell renal cell carcinoma identifies metabolic reprogramming associated with disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Hu,Shao-Gang Wang,Yaxin Hou,Zhaohui Chen,Lilong Liu,Ruizhi Li,Nisha Li,Lijie Zhou,Yu Yang,Liping Wang,Liang Wang,Xiong Yang,Yichen Lei,Changqi Deng,Yang Li,Zhiyao Deng,Yuhong Ding,Yingchun Kuang,Zhipeng Yao,Yang Xun,Fan Li,Heng Li,Jia Hu,Zheng Liu,Tao Wang,Yi Hao,Xuanmao Jiao,Wei Guan,Zhen Tao,Shancheng Ren,Ke Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Factor XIIIa antibody [EP3372]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Western blot - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Western blot - Anti-Factor XIIIa antibody [EP3372] (AB76105)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise