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AB218392

Anti-Factor XIIIa antibody [F13A1/1448]

4

(1 Review)

|

(1 Publication)

Mouse Monoclonal Factor XIIIa antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F13A1 aa 1-200.

View Alternative Names

F13A, F13A1, Coagulation factor XIII A chain, Coagulation factor XIIIa, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] (AB218392)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] (AB218392)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human histiocytoma tissue, labeling Factor XIIIa using ab218392 at 2 μg/mL.

Western blot - Anti-Factor XIIIa antibody [F13A1/1448] (AB218392)
  • WB

Supplier Data

Western blot - Anti-Factor XIIIa antibody [F13A1/1448] (AB218392)

All lanes:

Western blot - Anti-Factor XIIIa antibody [F13A1/1448] (ab218392) at 1 µg/mL

Lane 1:

Recombinant Human Factor XIIIa protein

Lane 2:

HeLa cell lysate

Predicted band size: 83 kDa

false

Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] (AB218392)
  • Protein Array

Unknown

Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] (AB218392)

ab218392 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F13A1/1448

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, Protein Array, WB

applications

Immunogen

Recombinant Fragment Protein within Human F13A1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P00488

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XIIIa also known as Factor 13a Factor XIII or Factor 13 is an enzyme that plays an important role in blood coagulation. Its mass is approximately 83 kDa. This enzyme is part of the transglutaminase family and is predominantly found in plasma and platelets. Factor XIIIa becomes active when thrombin and calcium ions cleave its A subunits converting it from an inactive zymogen known simply as Factor XIII or Factor 13 to its active form. The best assays for Factor XIIIa often measure its transglutaminase activity which facilitates the cross-linking of fibrin stabilizing blood clots.
Biological function summary

Factor XIIIa is important for hemostasis facilitating the final stages of blood clot formation by cross-linking fibrin polymers. This enzyme does not function in isolation; it forms a tetrameric complex with two A subunits and two B subunits. The active form Factor XIIIa catalyzes the formation of covalent bonds between glutamine and lysine residues in fibrin chains. This enhances the mechanical strength and resistance of the clot preventing premature degradation.

Pathways

Factor XIIIa is part of the coagulation pathway specifically involved in the stabilization of fibrin in the final steps of the cascade. It interacts closely with proteins like fibrinogen and thrombin as these proteins are essential in the conversion of Factor XIII to Factor XIIIa and in clot formation. Factor XIIIa also plays a role in the wound healing process by stabilizing the ECM (extracellular matrix) through cross-linking fibrin fibronectin and collagen.

Factor XIIIa deficiency can lead to bleeding diathesis a condition characterized by poor clot stability and prolonged bleeding. This deficiency is often congenital and involves reduced levels or activity of Factor XIII. Conversely overactivity or dysregulation of Factor XIIIa may contribute to thrombotic disorders where abnormal clot formation can lead to conditions like deep vein thrombosis. Clinically understanding Factor XIIIa interactions especially its functional relationships with fibrinogen and thrombin is critical in managing these bleeding and thrombotic disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
See full target information F13A1

Publications (1)

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iScience 27:110562 PubMed39175775

2024

WNT1-inducible signaling pathway protein 1 activation through C-X-C motif chemokine ligand 5/C-X-C chemokine receptor type 2/leukemia inhibitory factor/leukemia inhibitory factor receptor signaling promotes immunosuppression and neuroendocrine differentiation in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Hung Tsui,Chien-Liang Liu,Hsiu-Lien Yeh,Ming-Kun Liu,Chien-Hsiu Li,Wei-Hao Chen,Kuo-Ching Jiang,Han-Ru Li,Phan Vu Thuy Dung,Michael Hsiao,Wassim Abou-Kheir,Yen-Nien Liu
View all publications

Product promise

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