Mouse Monoclonal Factor XIIIa antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human F13A1 aa 1-200.
Images
![Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (AB218859), expandable thumbnail](https://content.abcam.com/products/images/factor-xiiia-antibody-f13a1-1448-bsa-and-azide-free-ab218859--protein-array-img141604.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (AB218859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (AB218859), expandable thumbnail](https://content.abcam.com/products/images/factor-xiiia-antibody-f13a1-1448-bsa-and-azide-free-ab218859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136837.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (AB218859)")
![Western blot - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (AB218859), expandable thumbnail](https://content.abcam.com/products/images/factor-xiiia-antibody-f13a1-1448-bsa-and-azide-free-ab218859--western-blot-img90909.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (AB218859)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human F13A1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P00488
Reactivity data
Select an application| IHC-P | WB | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes (Primary incubation for 30 min at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
7 products for Alternative Product
Target data
Function
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Alternative names
Coagulation factor XIII A chain, Coagulation factor XIIIa, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain, F13A, F13A1
Recommended products
Mouse Monoclonal Factor XIIIa antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human F13A1 aa 1-200.
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human F13A1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P00488
- Carrier free
Yes
- Clone number
F13A1/1448
- Purification technique
Affinity purification Protein A
- Light chain type
kappa
- Concentration
- Purification notes
ab218859 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor XIIIa also known as Factor 13a Factor XIII or Factor 13 is an enzyme that plays an important role in blood coagulation. Its mass is approximately 83 kDa. This enzyme is part of the transglutaminase family and is predominantly found in plasma and platelets. Factor XIIIa becomes active when thrombin and calcium ions cleave its A subunits converting it from an inactive zymogen known simply as Factor XIII or Factor 13 to its active form. The best assays for Factor XIIIa often measure its transglutaminase activity which facilitates the cross-linking of fibrin stabilizing blood clots.
- Biological function summary
Factor XIIIa is important for hemostasis facilitating the final stages of blood clot formation by cross-linking fibrin polymers. This enzyme does not function in isolation; it forms a tetrameric complex with two A subunits and two B subunits. The active form Factor XIIIa catalyzes the formation of covalent bonds between glutamine and lysine residues in fibrin chains. This enhances the mechanical strength and resistance of the clot preventing premature degradation.
- Pathways
Factor XIIIa is part of the coagulation pathway specifically involved in the stabilization of fibrin in the final steps of the cascade. It interacts closely with proteins like fibrinogen and thrombin as these proteins are essential in the conversion of Factor XIII to Factor XIIIa and in clot formation. Factor XIIIa also plays a role in the wound healing process by stabilizing the ECM (extracellular matrix) through cross-linking fibrin fibronectin and collagen.
- Associated diseases and disorders
Factor XIIIa deficiency can lead to bleeding diathesis a condition characterized by poor clot stability and prolonged bleeding. This deficiency is often congenital and involves reduced levels or activity of Factor XIII. Conversely overactivity or dysregulation of Factor XIIIa may contribute to thrombotic disorders where abnormal clot formation can lead to conditions like deep vein thrombosis. Clinically understanding Factor XIIIa interactions especially its functional relationships with fibrinogen and thrombin is critical in managing these bleeding and thrombotic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859), expandable thumbnail](https://content.abcam.com/products/images/factor-xiiia-antibody-f13a1-1448-bsa-and-azide-free-ab218859--protein-array-img141604.jpg "Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)")
Protein Array - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)
This data was produced with Anti-Factor XIIIa antibody [F13A1/1448] ab218392, the same antibody in a different formulation with BSA and Azide.
Anti-Factor XIIIa antibody [F13A1/1448] ab218392 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859), expandable thumbnail](https://content.abcam.com/products/images/factor-xiiia-antibody-f13a1-1448-bsa-and-azide-free-ab218859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136837.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human histiocytoma tissue labeling Factor XIIIa with ab218859 at 2 μg/ml.
![Western blot - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859), expandable thumbnail](https://content.abcam.com/products/images/factor-xiiia-antibody-f13a1-1448-bsa-and-azide-free-ab218859--western-blot-img90909.jpg "Western blot - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)")
Western blot - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)
All lanes: Western blot - Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859) at 1 µg/mL
Lane 1: Recombinant Human Factor XIIIa protein
Lane 2: HeLa cell lysate
Predicted band size: 83 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com