AB24533

Anti-FADD antibody

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(27 Publications)

Rabbit Polyclonal FADD antibody. Suitable for WB and reacts with Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human FADD aa 100-150.

View Alternative Names

MORT1, GIG3, FADD, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity

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Supplier Data

Western blot - Anti-FADD antibody (AB24533)

All lanes:

Western blot - Anti-FADD antibody (ab24533)

All lanes:

Jurkat cell lysates

Predicted band size: 23 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human FADD aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q13158

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Reactivity data

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3039 FADD Antibody. 3039-100 is the same size as the 100 µg size of ab24533.

FADD has a molecular weight of 23kDa with an apparent molecular weight of 28 kDa on SDS-PAGE.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FADD also known as Fas-Associated protein with Death Domain is an adaptor molecule with a molecular weight of approximately 23 kDa. It plays a critical role in the transmission of apoptotic signals. FADD is widely expressed in various tissues particularly in the thymus and immune system cells. This protein serves as a bridge linking death receptors like Fas and TNFR-1 with caspase activation pathways.

Biological function summary

FADD is essential in apoptosis where it assists in the assembly of the death-inducing signaling complex (DISC). Upon receptor activation FADD recruits procaspase-8 or -10 to DISC promoting their autocatalytic cleavage and activation. This leads to the subsequent cascade that results in cell apoptosis. FADD also plays a role in necroptosis and is involved in the immune response regulation highlighting its multifunctional nature in cellular processes.

Pathways

FADD integrates into the apoptotic and necroptotic pathways. In the apoptotic pathway it interacts closely with Fas a death receptor to promote caspase-8 activation. Additionally in the necroptotic pathway FADD associates with RIP1 and RIP3 contributing to an alternative form of programmed cell death. These interactions underline its significant role in controlling cell fate decisions.

Aberrations in FADD function are associated with cancer and autoimmune diseases. Overexpression or mutation of FADD can lead to unchecked cell proliferation or defective apoptosis contributing to cancer development. In autoimmune disorders improper regulation of FADD may disrupt immune tolerance and lead to systemic inflammation. Key proteins involved in these disease processes include caspase-8 and RIPK1 which interact with FADD in regulating cell death and survival mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors (PubMed : 16762833, PubMed : 19118384, PubMed : 20935634, PubMed : 23955153, PubMed : 24025841, PubMed : 7538907, PubMed : 9184224). The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed : 16762833, PubMed : 19118384, PubMed : 20935634, PubMed : 7538907, PubMed : 9184224). Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis (PubMed : 16762833). Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (PubMed : 21109225, PubMed : 24204270).

See full target information FADD

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Science translational medicine 14:eabj4375 PubMed36475903

2022

Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Ferreira-Gonzalez,Tak Yung Man,Hannah Esser,Rhona Aird,Alastair M Kilpatrick,Daniel Rodrigo-Torres,Nicholas Younger,Lara Campana,Victoria L Gadd,Benjamin Dwyer,Niya Aleksieva,Luke Boulter,Mark T Macmillan,Yinmiao Wang,Katie J Mylonas,David A Ferenbach,Timothy J Kendall,Wei-Yu Lu,Juan Carlos Acosta,Dominic Kurian,Stephen O'Neill,Gabriel C Oniscu,Jesus M Banales,Paul J Krimpenfort,Stuart J Forbes

American journal of cancer research 12:2118-2131 PubMed35693092

2022

Oral administration of TRAIL-inducing small molecule ONC201/TIC10 prevents intestinal polyposis in the mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Venkateshwar Madka,Arielle De La Cruz,Gopal Pathuri,Janani Panneerselvam,Yuting Zhang,Nicole Stratton,Sean Hacking,Niklas K Finnberg,Howard P Safran,Shizuko Sei,Elizabeth R Glaze,Robert Shoemaker,Jennifer T Fox,Alexander G Raufi,Wafik S El-Deiry,Chinthalapally V Rao

Cell biology and toxicology 39:237-258 PubMed34713381

2021

Sirt6 protects cardiomyocytes against doxorubicin-induced cardiotoxicity by inhibiting P53/Fas-dependent cell death and augmenting endogenous antioxidant defense mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Wu,Jie Lan,Lingyu Li,Xiaoxiao Wang,Mingming Tong,Li Fu,Yanjing Zhang,Jiayi Xu,Xuemei Chen,Hongying Chen,Ruli Li,Yao Wu,Juanjuan Xin,Xin Yan,He Li,Kunyue Xue,Xue Li,Caili Zhuo,Wei Jiang

Biomolecules 11: PubMed34356602

2021

Puerarin Attenuates Cadmium-Induced Neuronal Injury via Stimulating Cadmium Excretion, Inhibiting Oxidative Stress and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangquan Wen,Li Wang,Hui Zou,Jianhong Gu,Ruilong Song,Jianchun Bian,Yan Yuan,Zongping Liu

Oncology reports 45: PubMed33786627

2021

Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liang,Jianxin Ju

British journal of pharmacology : PubMed33434950

2021

M2 TAM-associated STAT3/NF-κB signalling suppression as major target of immunomodulatory therapy with PLGA-based nanocarriers and anti-PD-L1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rômulo S Cavalcante,Uta Ishikawa,Emanuell S Silva,Arnóbio A Silva Júnior,Aurigena A Araújo,Luis J Cruz,Alan B Chan,Raimundo F Araújo Júnior

The Journal of pharmacology and experimental therapeutics 377:86-99 PubMed33310816

2020

A Network Pharmacology-Based Analysis of the Protective Mechanism of Miao Medicine Xuemaitong Capsule Against Secondary Brain Damage in the Ischemic Area Surrounding Intracerebral Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Zhengyan Zeng,Haijun Wu

International journal of molecular medicine 45:1464-1476 PubMed32323821

2020

Circulating microRNA let‑7e is decreased in knee osteoarthritis, accompanied by elevated apoptosis and reduced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Feng,Chun Feng,Chang-Xing Wang,Dan-Yi Xu,Jun-Jie Chen,Jie-Feng Huang,Pan-Li Tan,Jin-Ming Shen

Pharmaceutics 12: PubMed32102251

2020

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide--Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Luiza C de S L Oliveira,Raimundo Fernandes de Araújo Júnior,Thaís Gomes de Carvalho,Alan B Chan,Timo Schomann,Filippo Tamburini,Lioe-Fee de Geus-Oei,Luis J Cruz

Theranostics 9:7961-7975 PubMed31695809

2019

miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Yudong Sun,Yu Xiao,Huiying Sun,Zhiqing Zhao,Jiang Zhu,Lei Zhang,Jian Dong,Tonglei Han,Qing Jing,Jian Zhou,Zaiping Jing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FADD antibody

Western blot - Anti-FADD antibody (AB24533)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

Product promise