AB124812

Anti-FADD antibody [EPR5030]

Name: Anti-FADD antibody [EPR5030] (ab124812) | Abcam
Brand: Abcam Limited
SKU: AB124812
Price: 590 USD
Availability: InStock
Rating: 4 (9 reviews)

RabMAb
Recombinant
What is this?

(9 Reviews)

(39 Publications)

Anti-FADD antibody [EPR5030] (ab124812) is a rabbit monoclonal antibody detecting FADD in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

Mort1, Fadd, FAS-associated death domain protein, FAS-associating death domain-containing protein, Mediator of receptor induced toxicity

6 Images

Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] (AB124812)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] (AB124812)

Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labelling FADD with purified ab124812 at 1/40 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (AB124812)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (AB124812)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FADD with purified ab124812 at 1/150 dilution (2.59 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] (AB124812)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] (AB124812)

Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling FADD with purified ab124812 at 1/50 dilution (7.8 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Lab

Immunoprecipitation - Anti-FADD antibody [EPR5030] (AB124812)

FADD was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg with ab124812 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab124812 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg. Lane 2 : NIH/3T3 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab124812 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Observed MW : 28 kDa. Exposure time : 41 secs.

Lab

Immunoprecipitation - Anti-FADD antibody [EPR5030] (AB124812)

FADD was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with ab124812 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab124812 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg. Lane 2 : RAW264.7 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab124812 in RAW264.7 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Observed MW : 28 kDa. Exposure time : 3 minutes.

Unknown

Western blot - Anti-FADD antibody [EPR5030] (AB124812)

All lanes:

Western blot - Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

false

Carrier free

Anti-FADD antibody [EPR5030] - BSA and Azide free
578 PE

PE Anti-FADD antibody [EPR5030]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-FADD antibody [EPR5030]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-FADD antibody [EPR5030]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-FADD antibody [EPR5030]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-FADD antibody [EPR5030]
660 APC

APC Anti-FADD antibody [EPR5030]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-FADD antibody [EPR5030]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5030

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-FADD antibody [EPR5030] (ab124812) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

What is the molecular weight of FADD?
Anti-FADD [EPR5030] (ab124812) specifically detects a band for FADD (UniProt: Q61160) at a molecular weight of 23kDa.

Trusted by the scientific community
Anti-FADD [EPR5030] (ab124812) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-FADD [EPR5030] (ab124812) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5030] also available for your convenience: ab124812, PE - ab211700, Carrier free - ab229444, APC - ab317936, Alexa Fluor® 488 - ab317976, Alexa Fluor® 594 - ab318017, Alexa Fluor® 555 - ab318059, Alexa Fluor® 647 - ab318101, Alexa Fluor® 750 - ab320980

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FADD also known as Fas-Associated protein with Death Domain is an adaptor molecule with a molecular weight of approximately 23 kDa. It plays a critical role in the transmission of apoptotic signals. FADD is widely expressed in various tissues particularly in the thymus and immune system cells. This protein serves as a bridge linking death receptors like Fas and TNFR-1 with caspase activation pathways.

Biological function summary

FADD is essential in apoptosis where it assists in the assembly of the death-inducing signaling complex (DISC). Upon receptor activation FADD recruits procaspase-8 or -10 to DISC promoting their autocatalytic cleavage and activation. This leads to the subsequent cascade that results in cell apoptosis. FADD also plays a role in necroptosis and is involved in the immune response regulation highlighting its multifunctional nature in cellular processes.

Pathways

FADD integrates into the apoptotic and necroptotic pathways. In the apoptotic pathway it interacts closely with Fas a death receptor to promote caspase-8 activation. Additionally in the necroptotic pathway FADD associates with RIP1 and RIP3 contributing to an alternative form of programmed cell death. These interactions underline its significant role in controlling cell fate decisions.

Aberrations in FADD function are associated with cancer and autoimmune diseases. Overexpression or mutation of FADD can lead to unchecked cell proliferation or defective apoptosis contributing to cancer development. In autoimmune disorders improper regulation of FADD may disrupt immune tolerance and lead to systemic inflammation. Key proteins involved in these disease processes include caspase-8 and RIPK1 which interact with FADD in regulating cell death and survival mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.

See full target information Fadd

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Computational and structural biotechnology journal 27:3066-3078 PubMed40697880

2025

Anticancer effects and mechanisms of , and on human lung carcinoma and hepatoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhen Li,Woonghee Kim,Han Jin,Hong Yang,Xiangtai Kong,Xiya Song,Hasan Turkez,Yuefeng Bi,Chengxue Pan,Ling Fu,Hongmin Liu,Mathias Uhlen,Cheng Zhang,Adil Mardinoglu

Nature communications 16:1890 PubMed39987261

2025

Cooperation of TRADD- and RIPK1-dependent cell death pathways in maintaining intestinal homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyu Sun,Jianyu Ye,Weimin Sun,Libo Jiang,Bing Shan,Mengmeng Zhang,Jingyi Xu,Wanjin Li,Jianping Liu,Hongyang Jing,Tian Zhang,Meiling Hou,Cen Xie,Rongling Wu,Heling Pan,Junying Yuan

The FEBS journal 292:1972-1990 PubMed39827378

2025

TNFAIP3-interacting protein 1 (ABIN-1) negatively regulates caspase-8/FADD-dependent pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyi Li,Daoyong Wang,Zhenyi Su,Xiaohua Mao

Nature communications 15:3791 PubMed38710704

2024

Deciphering DED assembly mechanisms in FADD-procaspase-8-cFLIP complexes regulating apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yu Yang,Chia-I Lien,Yi-Chun Tseng,Yi-Fan Tu,Arkadiusz W Kulczyk,Yen-Chen Lu,Yin-Ting Wang,Tsung-Wei Su,Li-Chung Hsu,Yu-Chih Lo,Su-Chang Lin

Proceedings of the National Academy of Sciences of the United States of America 120:e2308079120 PubMed37733743

2023

Small molecule activators of TAK1 promotes its activity-dependent ubiquitination and TRAIL-mediated tumor cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Weimin Sun,Guowei Wu,Xinyu Tian,Chunting Qi,Jingli Liu,Yilun Tong,Mengmeng Zhang,Jiayang Gao,Ze Cao,Yuchao Zhang,Zhijun Liu,Xiaoxu Tian,Ping Wu,Chao Peng,Jingwen Li,Li Tan,Bing Shan,Jianping Liu,Ying Li,Junying Yuan

Science advances 9:eadg2829 PubMed37494451

2023

Cleavage of cFLIP restrains cell death during viral infection and tissue injury and favors tissue repair.

Applications

Unspecified application

Species

Unspecified reactive species

Kristel Martinez Lagunas,Deniz Pinar Savcigil,Matea Zrilic,Carlos Carvajal Fraile,Andrew Craxton,Emily Self,Iratxe Uranga-Murillo,Diego de Miguel,Maykel Arias,Sebastian Willenborg,Michael Piekarek,Marie Christine Albert,Kalvin Nugraha,Ina Lisewski,Erika Janakova,Natalia Igual,Wulf Tonnus,Ximena Hildebrandt,Mohammed Ibrahim,Marlies Ballegeer,Xavier Saelens,Andrew Kueh,Pascal Meier,Andreas Linkermann,Julian Pardo,Sabine Eming,Henning Walczak,Marion MacFarlane,Nieves Peltzer,Alessandro Annibaldi

Breast cancer research : BCR 25:75 PubMed37365643

2023

Transcriptionally regulated miR-26a-5p may act as BRCAness in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Lianqiu Lv,Renjing Zheng,Rong Xie,Yuanhang Yu,Han Liao,Jianying Chen,Bo Zhang

Nature communications 14:2715 PubMed37169760

2023

UDP-glucuronate metabolism controls RIPK1-driven liver damage in nonalcoholic steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Na Zhang,Jing Xing,Shuhua Zhang,Yulu Chen,Daichao Xu,Jinyang Gu

Nature communications 13:7153 PubMed36414671

2022

SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjie Yan,Tao Zhang,Kai Wang,Zezhao Chen,Yuanxin Yang,Bing Shan,Qi Sun,Mengmeng Zhang,Yichi Zhang,Yedan Zhong,Nan Liu,Jinyang Gu,Daichao Xu

Cell death & disease 13:773 PubMed36071040

2022

PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIP to promote TNFα-mediated cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Guowei Wu,Dekang Li,Wei Liang,Weimin Sun,Xingxing Xie,Yilun Tong,Bing Shan,Mengmeng Zhang,Xiaojuan Lu,Junying Yuan,Ying Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FADD antibody [EPR5030]

Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] (AB124812)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (AB124812)

Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] (AB124812)

Immunoprecipitation - Anti-FADD antibody [EPR5030] (AB124812)

Immunoprecipitation - Anti-FADD antibody [EPR5030] (AB124812)

Western blot - Anti-FADD antibody [EPR5030] (AB124812)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

Product promise