Rabbit Recombinant Monoclonal FADD antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra), IP and reacts with Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunocytochemistry-immunofluorescence-img168887.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444)")
![Western blot - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--western-blot-img174048.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178111.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444)")
![Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--flow-cytometry-intracellular-img183268.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444)")
![Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunoprecipitation-img400147.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (AB229444)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Mouse | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Alternative names
Mort1, Fadd, FAS-associated death domain protein, FAS-associating death domain-containing protein, Mediator of receptor induced toxicity
Recommended products
Rabbit Recombinant Monoclonal FADD antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra), IP and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5030
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab229444 is the carrier-free version of Anti-FADD antibody [EPR5030] ab124812.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FADD also known as Fas-Associated protein with Death Domain is an adaptor molecule with a molecular weight of approximately 23 kDa. It plays a critical role in the transmission of apoptotic signals. FADD is widely expressed in various tissues particularly in the thymus and immune system cells. This protein serves as a bridge linking death receptors like Fas and TNFR-1 with caspase activation pathways.
- Biological function summary
FADD is essential in apoptosis where it assists in the assembly of the death-inducing signaling complex (DISC). Upon receptor activation FADD recruits procaspase-8 or -10 to DISC promoting their autocatalytic cleavage and activation. This leads to the subsequent cascade that results in cell apoptosis. FADD also plays a role in necroptosis and is involved in the immune response regulation highlighting its multifunctional nature in cellular processes.
- Pathways
FADD integrates into the apoptotic and necroptotic pathways. In the apoptotic pathway it interacts closely with Fas a death receptor to promote caspase-8 activation. Additionally in the necroptotic pathway FADD associates with RIP1 and RIP3 contributing to an alternative form of programmed cell death. These interactions underline its significant role in controlling cell fate decisions.
- Associated diseases and disorders
Aberrations in FADD function are associated with cancer and autoimmune diseases. Overexpression or mutation of FADD can lead to unchecked cell proliferation or defective apoptosis contributing to cancer development. In autoimmune disorders improper regulation of FADD may disrupt immune tolerance and lead to systemic inflammation. Key proteins involved in these disease processes include caspase-8 and RIPK1 which interact with FADD in regulating cell death and survival mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunocytochemistry-immunofluorescence-img168887.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
This data was developed using Anti-FADD antibody [EPR5030] ab124812, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling FADD with purified Anti-FADD antibody [EPR5030] ab124812 at 1/50 dilution (7.8 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--western-blot-img174048.jpg "Western blot - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Western blot - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
All lanes: Western blot - Anti-FADD antibody [EPR5030] (Anti-FADD antibody [EPR5030] ab124812) at 1/1000 dilution
All lanes: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178111.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
This data was developed using Anti-FADD antibody [EPR5030] ab124812, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FADD with purified Anti-FADD antibody [EPR5030] ab124812 at 1/150 dilution (2.59 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.![Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--flow-cytometry-intracellular-img183268.jpg "Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
This data was developed using Anti-FADD antibody [EPR5030] ab124812, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labelling FADD with purified Anti-FADD antibody [EPR5030] ab124812 at 1/40 dilution (10 �g/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunoprecipitation-img400147.jpg "Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
FADD was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg with Anti-FADD antibody [EPR5030] ab124812 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using Anti-FADD antibody [EPR5030] ab124812 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 μg.
Lane 2: NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FADD antibody [EPR5030] ab124812 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Observed MW : 28 kDa.
Exposure time: 41 secs.
This data was developed using Anti-FADD antibody [EPR5030] ab124812, the same antibody clone in a different buffer formulation.![Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunoprecipitation-img400148.jpg "Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
FADD was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with Anti-FADD antibody [EPR5030] ab124812 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using Anti-FADD antibody [EPR5030] ab124812 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg.
Lane 2: RAW264.7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FADD antibody [EPR5030] ab124812 in RAW264.7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Observed MW : 28 kDa.
Exposure time: 3 minutes.
This data was developed using Anti-FADD antibody [EPR5030] ab124812, the same antibody clone in a different buffer formulation.
![Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444), expandable thumbnail](https://content.abcam.com/products/images/fadd-antibody-epr5030-bsa-and-azide-free-ab229444--immunoprecipitation-img400148.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
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