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Rabbit Recombinant Monoclonal FADS1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 19 publications.

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Images

Western blot - Anti-FADS1 antibody [EPR6898] (AB126706), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-FADS1 antibody [EPR6898] (AB126706), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS1 antibody [EPR6898] (AB126706), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FADS1 antibody [EPR6898] (AB126706), expandable thumbnail
  • OI-RD Scanning - Anti-FADS1 antibody [EPR6898] (AB126706), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted
Predicted
Rat
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Isoform 1. Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (PubMed:10601301, PubMed:10769175). As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (By similarity). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity). Isoform 2. Does not exhibit any catalytic activity toward 20:3n-6, but it may enhance FADS2 activity.

Alternative names

FADSD5, FADS1, Acyl-CoA (8-3)-desaturase, Delta(5) fatty acid desaturase, Fatty acid desaturase 1, D5D, Delta(5) desaturase, Delta-5 desaturase

Recommended products

Rabbit Recombinant Monoclonal FADS1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 19 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6898
Purification technique
Affinity purification Protein A
Dissociation constant
7.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FADS1 also known as fatty acid desaturase 1 is an enzyme that introduces double bonds into fatty acid chains. This process is critical in polyunsaturated fatty acid biosynthesis. FADS1 has a molecular mass of approximately 52 kDa. The enzyme is expressed in a variety of tissues with significant presence in the liver where it plays an important role in lipid metabolism. The expression levels of FADS1 can vary based on dietary intake and the body's metabolic needs.

Biological function summary

FADS1 catalyzes the desaturation of specific fatty acids by converting them into more unsaturated forms. It acts within the endoplasmic reticulum of cells and can function as part of a larger enzyme complex. The activity of FADS1 influences the levels of essential fatty acids like arachidonic acid and eicosapentaenoic acid which affect cellular membrane structure and signaling. This enzyme's action plays a significant role in creating lipid mediators that are important for anti-inflammatory processes and cellular communication.

Pathways

The activity of FADS1 is integral to the omega-3 and omega-6 fatty acid metabolic pathways. These pathways have implications for cellular homeostasis and are related to energy balance and inflammatory responses. Enzymes such as FADS2 are closely related through these pathways operating in tandem to progressively desaturate and elongate fatty acids. The balance between FADS1 and FADS2 activities influences the product profiles of these pathways thereby affecting overall fatty acid composition in the body.

Associated diseases and disorders

FADS1 has links to metabolic disorders including cardiovascular disease and dyslipidemia. Aberrations in FADS1 activity can lead to imbalanced fatty acid levels which may contribute to these conditions. There are studies suggesting its connection to inflammatory diseases through altered eicosanoid production impacting proteins like cyclooxygenase. Understanding the variations in FADS1 function and expression could aid in developing therapeutic strategies targeting metabolic health and inflammatory conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-FADS1 antibody [EPR6898] (ab126706), expandable thumbnail

    Western blot - Anti-FADS1 antibody [EPR6898] (ab126706)

    All lanes: Western blot - Anti-FADS1 antibody [EPR6898] (ab126706) at 1/1000 dilution

    Lane 1: Human fetal lung lysate at 10 µg

    Lane 2: Human fetal liver lysate at 10 µg

    Lane 3: Human fetal brain lysate at 10 µg

    Lane 4: HepG2 lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa

  • Flow Cytometry (Intracellular) - Anti-FADS1 antibody [EPR6898] (ab126706), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-FADS1 antibody [EPR6898] (ab126706)

    Overlay histogram showing HepG2 cells stained with ab126706 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126706, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS1 antibody [EPR6898] (ab126706), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS1 antibody [EPR6898] (ab126706)

    ab126706, at 1/100 dilution, staining FADS1 in Paraffin-embedded Human heart tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FADS1 antibody [EPR6898] (ab126706), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FADS1 antibody [EPR6898] (ab126706)

    ab126706, at 1/100 dilution, staining FADS1 in A549 cells by Immunofluorescence.

  • OI-RD Scanning - Anti-FADS1 antibody [EPR6898] (ab126706), expandable thumbnail

    OI-RD Scanning - Anti-FADS1 antibody [EPR6898] (ab126706)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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