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AB262943

Anti-FADS2 antibody

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Rabbit Polyclonal FADS2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FADS2 aa 1-150.

View Alternative Names

Acyl-CoA 6-desaturase, Delta(6) fatty acid desaturase, Fatty acid desaturase 2, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB262943)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB262943)

Paraffin-embedded Human adrenal gland tissue stained for FADS2 using ab262943 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FADS2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O95864

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FADS2 also known as Fatty Acid Desaturase 2 or FABRED27 functions as an enzyme involved in creating double bonds in specific fatty acids. It holds a molecular weight of approximately 52 kDa. Researchers find this enzyme prominently expressed in liver tissue and it plays an essential role in the process of lipid metabolism.
Biological function summary

FADS2 contributes to the metabolism of polyunsaturated fatty acids by introducing double bonds specifically within the fatty acid synthetic pathway. It does not function as part of a larger complex but operates independently. Through its enzymatic action it influences the production of essential fatty acids such as arachidonic acid and eicosapentaenoic acid which are important for maintaining normal cellular function.

Pathways

FADS2 plays an integral part within both the fatty acid biosynthesis and fatty acid metabolism pathways. It closely relates with other enzymes such as acyl-CoA synthetase and elongation enzymes which work together to metabolize various fats within the body. These pathways help maintain cellular homeostasis by regulating levels of essential long-chain polyunsaturated fatty acids.

Alterations in FADS2 activity have connections to metabolic disorders like obesity and cardiovascular disease. Aberrant expression of FADS2 can influence lipid profiles in these conditions potentially exacerbating disease outcomes. FADS2 also interacts with other proteins such as SCD1 (Stearoyl-CoA Desaturase 1) within these disorders further highlighting its role in lipid metabolism and associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18 : 2n-6) and alpha-linolenic acid (ALA) (18 : 3n-3) precursors, acting as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18 : 2n-6) and ALA (18 : 3n-3) into gamma-linoleate (GLA) (18 : 3n-6) and stearidonate (18 : 4n-3), respectively (PubMed : 12713571). Subsequently, in the biosynthetic pathway of HUFA n-3 series, it desaturates tetracosapentaenoate (24 : 5n-3) to tetracosahexaenoate (24 : 6n-3), which is then converted to docosahexaenoate (DHA)(22 : 6n-3), an important lipid for nervous system function (By similarity). Desaturates hexadecanate (palmitate) to produce 6Z-hexadecenoate (sapienate), a fatty acid unique to humans and major component of human sebum, that has been implicated in the development of acne and may have potent antibacterial activity (PubMed : 12713571). It can also desaturate (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 6 generating (6Z,11E)-octadecadienoate (By similarity). In addition to Delta-6 activity, this enzyme exhibits Delta-8 activity with slight biases toward n-3 fatty acyl-CoA substrates (By similarity).
See full target information FADS2
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