Rabbit Recombinant Monoclonal FADS2 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.
Images
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421027.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)")
![Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--flow-cytometry-intracellular-img421164.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)")
![Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunocytochemistry-immunofluorescence-img421149.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421115.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421114.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested | Expected | Expected |
| Rat | Tested | Tested | Tested | Expected | Expected |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Involved in the biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors, acting as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:12713571). Subsequently, in the biosynthetic pathway of HUFA n-3 series, it desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (By similarity). Desaturates hexadecanate (palmitate) to produce 6Z-hexadecenoate (sapienate), a fatty acid unique to humans and major component of human sebum, that has been implicated in the development of acne and may have potent antibacterial activity (PubMed:12713571). It can also desaturate (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 6 generating (6Z,11E)-octadecadienoate (By similarity). In addition to Delta-6 activity, this enzyme exhibits Delta-8 activity with slight biases toward n-3 fatty acyl-CoA substrates (By similarity).
Alternative names
Acyl-CoA 6-desaturase, Delta(6) fatty acid desaturase, Fatty acid desaturase 2, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2
Recommended products
Rabbit Recombinant Monoclonal FADS2 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27357-82
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314318 is the carrier-free version of Anti-FADS2 antibody [EPR27357-82] ab314317.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FADS2 also known as Fatty Acid Desaturase 2 or FABRED27 functions as an enzyme involved in creating double bonds in specific fatty acids. It holds a molecular weight of approximately 52 kDa. Researchers find this enzyme prominently expressed in liver tissue and it plays an essential role in the process of lipid metabolism.
- Biological function summary
FADS2 contributes to the metabolism of polyunsaturated fatty acids by introducing double bonds specifically within the fatty acid synthetic pathway. It does not function as part of a larger complex but operates independently. Through its enzymatic action it influences the production of essential fatty acids such as arachidonic acid and eicosapentaenoic acid which are important for maintaining normal cellular function.
- Pathways
FADS2 plays an integral part within both the fatty acid biosynthesis and fatty acid metabolism pathways. It closely relates with other enzymes such as acyl-CoA synthetase and elongation enzymes which work together to metabolize various fats within the body. These pathways help maintain cellular homeostasis by regulating levels of essential long-chain polyunsaturated fatty acids.
- Associated diseases and disorders
Alterations in FADS2 activity have connections to metabolic disorders like obesity and cardiovascular disease. Aberrant expression of FADS2 can influence lipid profiles in these conditions potentially exacerbating disease outcomes. FADS2 also interacts with other proteins such as SCD1 (Stearoyl-CoA Desaturase 1) within these disorders further highlighting its role in lipid metabolism and associated diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
13 product images
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421027.jpg "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
FADS2 Western blot staining using rabbit Anti-FADS2 antibody
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.All lanes: Western blot - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeti FADS2 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa
Exposure time: 81s
![Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--flow-cytometry-intracellular-img421164.jpg "Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling FADS2 with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunocytochemistry-immunofluorescence-img421149.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling FADS2 with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/50 (10.26 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing the cytoplasmic in HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421115.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling FADS2 with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: Weak staining on human lung. The section was incubated with Anti-FADS2 antibody [EPR27357-82] ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421114.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling FADS2 with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat liver. The section was incubated with Anti-FADS2 antibody [EPR27357-82] ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421113.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling FADS2 with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with Anti-FADS2 antibody [EPR27357-82] ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421112.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FADS2 with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID: 32452400). The section was incubated with Anti-FADS2 antibody [EPR27357-82] ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunoprecipitation-img421060.jpg "Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
FADS2 was immunoprecipitated from 0.35 mg Rat liver tissue lysate with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat liver tissue lysate
Lane 2: Anti-FADS2 antibody [EPR27357-82] ab314317 IP in Rat liver tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FADS2 antibody [EPR27357-82] ab314317 in rat liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/30 dilution
All lanes: Rat liver tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
![Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunoprecipitation-img421059.jpg "Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
FADS2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: Anti-FADS2 antibody [EPR27357-82] ab314317 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FADS2 antibody [EPR27357-82] ab314317 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
![Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunoprecipitation-img421058.jpg "Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
FADS2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-FADS2 antibody [EPR27357-82] ab314317 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-FADS2 antibody [EPR27357-82] ab314317 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FADS2 antibody [EPR27357-82] ab314317 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/30 dilution
All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421030.jpg "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: lung tissue(PMID: 12713571)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/1000 dilution
Lane 1: Human lung tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse lung tissue lysate at 20 µg
Lane 5: Rat lung tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa
Exposure time: 180s
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421029.jpg "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human FADS1 and FADS3.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilutionAll lanes: Western blot - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human FADS3 expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human FADS1 expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human FADS2 expression vector containing a His-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa
Exposure time: 180s
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421028.jpg "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)
This data was developed using Anti-FADS2 antibody [EPR27357-82] ab314317, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression of FADS2 is downregulated in response to sorafenib treatment (PMID: 31199678).
The blot was developed using a high sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-FADS2 antibody [EPR27357-82] (Anti-FADS2 antibody [EPR27357-82] ab314317) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HeLa treated with 40 uM sorafenib for 6 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 45 kDa
Exposure time: 70s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421113.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421112.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunoprecipitation-img421060.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunoprecipitation-img421059.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--immunoprecipitation-img421058.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421030.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421029.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
![Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318), expandable thumbnail](https://content.abcam.com/products/images/fads2-antibody-epr27357-82-bsa-and-azide-free-ab314318--western-blot-img421028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (ab314318)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com