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AB314318

Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free

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Rabbit Recombinant Monoclonal FADS2 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.

View Alternative Names

Acyl-CoA 6-desaturase, Delta(6) fatty acid desaturase, Fatty acid desaturase 2, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human lung tissue labeling FADS2 with ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : Weak staining on human lung. The section was incubated with ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FADS2 with ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID : 32452400). The section was incubated with ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling FADS2 with ab314317 at 1/50 (10.26 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing the cytoplasmic in HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling FADS2 with ab314317 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IP

Supplier Data

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. FADS2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab314317 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314317 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : ab314317 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314317 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling FADS2 with ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat liver. The section was incubated with ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling FADS2 with ab314317 at 1/2000 (0.262 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with ab314317 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IP

Supplier Data

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. FADS2 was immunoprecipitated from 0.35 mg Rat liver tissue lysate with ab314317 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314317 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat liver tissue lysate Lane 2 : ab314317 IP in Rat liver tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314317 in rat liver tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/30 dilution

All lanes:

Rat liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • IP

Supplier Data

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. FADS2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab314317 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314317 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab314317 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314317 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • WB

Supplier Data

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : lung tissue(PMID : 12713571) In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse lung tissue lysate at 20 µg

Lane 5:

Rat lung tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 180s

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • WB

Supplier Data

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeti FADS2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 81s

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • WB

Supplier Data

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression of FADS2 is downregulated in response to sorafenib treatment (PMID : 31199678). The blot was developed using a high sensitivity ECL substrate. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa treated with 40 uM sorafenib for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

true

Exposure time: 70s

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)
  • WB

Supplier Data

Western blot - Anti-FADS2 antibody [EPR27357-82] - BSA and Azide free (AB314318)

This data was developed using ab314317, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human FADS1 and FADS3. In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution

All lanes:

Western blot - Anti-FADS2 antibody [EPR27357-82] (<a href='/en-us/products/primary-antibodies/fads2-antibody-epr27357-82-ab314317'>ab314317</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human FADS3 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human FADS1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human FADS2 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 51 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27357-82

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, ICC/IF, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab314318 is the carrier-free version of ab314317.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FADS2 also known as Fatty Acid Desaturase 2 or FABRED27 functions as an enzyme involved in creating double bonds in specific fatty acids. It holds a molecular weight of approximately 52 kDa. Researchers find this enzyme prominently expressed in liver tissue and it plays an essential role in the process of lipid metabolism.
Biological function summary

FADS2 contributes to the metabolism of polyunsaturated fatty acids by introducing double bonds specifically within the fatty acid synthetic pathway. It does not function as part of a larger complex but operates independently. Through its enzymatic action it influences the production of essential fatty acids such as arachidonic acid and eicosapentaenoic acid which are important for maintaining normal cellular function.

Pathways

FADS2 plays an integral part within both the fatty acid biosynthesis and fatty acid metabolism pathways. It closely relates with other enzymes such as acyl-CoA synthetase and elongation enzymes which work together to metabolize various fats within the body. These pathways help maintain cellular homeostasis by regulating levels of essential long-chain polyunsaturated fatty acids.

Alterations in FADS2 activity have connections to metabolic disorders like obesity and cardiovascular disease. Aberrant expression of FADS2 can influence lipid profiles in these conditions potentially exacerbating disease outcomes. FADS2 also interacts with other proteins such as SCD1 (Stearoyl-CoA Desaturase 1) within these disorders further highlighting its role in lipid metabolism and associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18 : 2n-6) and alpha-linolenic acid (ALA) (18 : 3n-3) precursors, acting as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18 : 2n-6) and ALA (18 : 3n-3) into gamma-linoleate (GLA) (18 : 3n-6) and stearidonate (18 : 4n-3), respectively (PubMed : 12713571). Subsequently, in the biosynthetic pathway of HUFA n-3 series, it desaturates tetracosapentaenoate (24 : 5n-3) to tetracosahexaenoate (24 : 6n-3), which is then converted to docosahexaenoate (DHA)(22 : 6n-3), an important lipid for nervous system function (By similarity). Desaturates hexadecanate (palmitate) to produce 6Z-hexadecenoate (sapienate), a fatty acid unique to humans and major component of human sebum, that has been implicated in the development of acne and may have potent antibacterial activity (PubMed : 12713571). It can also desaturate (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 6 generating (6Z,11E)-octadecadienoate (By similarity). In addition to Delta-6 activity, this enzyme exhibits Delta-8 activity with slight biases toward n-3 fatty acyl-CoA substrates (By similarity).
See full target information FADS2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com