Rabbit Polyclonal FADS3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FADS3.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human FADS3. Database link Q9Y5Q0
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Baboon | Predicted |
| Chimpanzee | Predicted |
| Cynomolgus monkey | Predicted |
| Pig | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base that comprises mammalian ceramides is sphing-4-enine (sphingosine or SPH) which has a trans (E) desaturation at carbon 4 (PubMed:31862735, PubMed:31916624). FADS3 is a desaturase that introduces a cis (Z) double bond between carbon 14 and carbon 15 of the sphingoid base (also known as long chain base, LCB), producing LCBs such as sphinga-4,14-dienine (SPD, d18:2(4E,14Z)) from SPH (PubMed:31862735, PubMed:31916624, PubMed:37209771). Prefers SPH-containing ceramides (N-acylsphing-4-enines) as substrates (PubMed:31862735, PubMed:31916624, PubMed:37209771). Capable of metabolizing also the SPH in its free form (PubMed:31862735). SPD ceramides occur widely in mammalian tissues and cells (PubMed:31916624). Due to their unusual structure containing a cis double bond, SPD ceramides may have an opposite, negative role in lipid microdomain formation relative to conventional ceramides (PubMed:31916624). Could be involved in the detoxification of 1-deoxy sphingolipids, by desaturating the cytotoxic 1-deoxysphinganine (1-deoxySA, m18:0), produced under pathological conditions, to 1-deoxysphingenine (1-deoxysphingosine, 1-deoxySO, m18:1) (Probable). Although prefers SPH-containing ceramides (N-acylsphing-4-enines) as substrates, it also exhibits activity toward dihydrosphingosine-containing CERs (N-acylsphinganines) and produces 14Z-SPH-containing sphingolipids,which can be found in patients with DEGS1 mutations (PubMed:37209771). Its desaturase mechanism involves an electron transfer facilitated by cytochrome b5 (PubMed:37209771). FADS3 also acts as a methyl-end fatty acyl coenzyme A (CoA) desaturase that introduces a cis double bond between the preexisting double bond and the terminal methyl group of the fatty acyl chain (By similarity). Desaturates (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 13 to generate (11E,13Z)-octadecadienoate (also known as conjugated linoleic acid 11E,13Z-CLA) (By similarity).
Alternative names
CYB5RP, FADS3, Fatty acid desaturase 3, Delta(13) fatty acid desaturase, Delta(13) desaturase
Recommended products
Rabbit Polyclonal FADS3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FADS3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human FADS3. Database link Q9Y5Q0
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FADS3 also known as fatty acid desaturase 3 is an enzyme that plays a role in the mechanical conversion of saturated fatty acids into unsaturated fatty acids through the introduction of double bonds. This enzyme is part of the desaturase family and has a molecular mass of approximately 53 kDa. FADS3 is expressed in various tissues including the liver brain and adipose tissue indicating its involvement in lipid metabolism and brain function.
- Biological function summary
The enzyme FADS3 affects the lipid biosynthesis pathway influencing the structure and function of cell membranes by altering the unsaturation levels of fatty acids. While FADS3 operates independently it shares mechanistic similarities with FADS1 and FADS2 which are part of the desaturase family. FADS3 primarily impacts the composition of lipids contributing to complex lipid pathways and influencing cellular signaling.
- Pathways
FADS3 is involved in essential lipid metabolic and signaling pathways. It contributes to the biosynthesis of polyunsaturated fatty acids (PUFAs) critical components of eicosanoid pathways which regulate inflammation and immunity. In these pathways FADS3 interacts indirectly with other fatty acid desaturases like FADS1 and FADS2 which sequentially catalyze steps in the PUFA biosynthesis pathway influencing overall lipid homeostasis.
- Associated diseases and disorders
FADS3 has connections to metabolic and neurological disorders. Its role in lipid metabolism aligns FADS3 with conditions like obesity and schizophrenia where lipid membrane composition may be disrupted. FADS3 indirectly affects conditions linked with FADS1 and FADS2 known to associate with altered PUFA levels in these disorders highlighting its potential as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-FADS3 antibody (ab169342)
All lanes: Western blot - Anti-FADS3 antibody (ab169342) at 1 µg/mL
Lane 1: FADS3-transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
Developed using the ECL technique.
Predicted band size: 51 kDa
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