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AB232810

Anti-FADS3 antibody

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(1 Publication)

Rabbit Polyclonal FADS3 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FADS3 aa 300 to C-terminus.

View Alternative Names

CYB5RP, FADS3, Fatty acid desaturase 3, Delta(13) fatty acid desaturase, Delta(13) desaturase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS3 antibody (AB232810)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS3 antibody (AB232810)

Formalin-fixed, paraffin-embedded human lung tissue stained for FADS3 using ab232810 at 20μg/ml in immunohistochemical analysis.

Western blot - Anti-FADS3 antibody (AB232810)
  • WB

Supplier Data

Western blot - Anti-FADS3 antibody (AB232810)

All lanes:

Western blot - Anti-FADS3 antibody (ab232810) at 0.2 µg/mL

All lanes:

Recombinant human FADS3

Predicted band size: 51 kDa

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Western blot - Anti-FADS3 antibody (AB232810)
  • WB

Supplier Data

Western blot - Anti-FADS3 antibody (AB232810)

All lanes:

Western blot - Anti-FADS3 antibody (ab232810) at 2 µg/mL

All lanes:

Pig stomach tissue lysate

Predicted band size: 51 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FADS3 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5Q0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FADS3 also known as fatty acid desaturase 3 is an enzyme that plays a role in the mechanical conversion of saturated fatty acids into unsaturated fatty acids through the introduction of double bonds. This enzyme is part of the desaturase family and has a molecular mass of approximately 53 kDa. FADS3 is expressed in various tissues including the liver brain and adipose tissue indicating its involvement in lipid metabolism and brain function.
Biological function summary

The enzyme FADS3 affects the lipid biosynthesis pathway influencing the structure and function of cell membranes by altering the unsaturation levels of fatty acids. While FADS3 operates independently it shares mechanistic similarities with FADS1 and FADS2 which are part of the desaturase family. FADS3 primarily impacts the composition of lipids contributing to complex lipid pathways and influencing cellular signaling.

Pathways

FADS3 is involved in essential lipid metabolic and signaling pathways. It contributes to the biosynthesis of polyunsaturated fatty acids (PUFAs) critical components of eicosanoid pathways which regulate inflammation and immunity. In these pathways FADS3 interacts indirectly with other fatty acid desaturases like FADS1 and FADS2 which sequentially catalyze steps in the PUFA biosynthesis pathway influencing overall lipid homeostasis.

FADS3 has connections to metabolic and neurological disorders. Its role in lipid metabolism aligns FADS3 with conditions like obesity and schizophrenia where lipid membrane composition may be disrupted. FADS3 indirectly affects conditions linked with FADS1 and FADS2 known to associate with altered PUFA levels in these disorders highlighting its potential as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base that comprises mammalian ceramides is sphing-4-enine (sphingosine or SPH) which has a trans (E) desaturation at carbon 4 (PubMed : 31862735, PubMed : 31916624). FADS3 is a desaturase that introduces a cis (Z) double bond between carbon 14 and carbon 15 of the sphingoid base (also known as long chain base, LCB), producing LCBs such as sphinga-4,14-dienine (SPD, d18 : 2(4E,14Z)) from SPH (PubMed : 31862735, PubMed : 31916624, PubMed : 37209771). Prefers SPH-containing ceramides (N-acylsphing-4-enines) as substrates (PubMed : 31862735, PubMed : 31916624, PubMed : 37209771). Capable of metabolizing also the SPH in its free form (PubMed : 31862735). SPD ceramides occur widely in mammalian tissues and cells (PubMed : 31916624). Due to their unusual structure containing a cis double bond, SPD ceramides may have an opposite, negative role in lipid microdomain formation relative to conventional ceramides (PubMed : 31916624). Could be involved in the detoxification of 1-deoxy sphingolipids, by desaturating the cytotoxic 1-deoxysphinganine (1-deoxySA, m18 : 0), produced under pathological conditions, to 1-deoxysphingenine (1-deoxysphingosine, 1-deoxySO, m18 : 1) (Probable). Although prefers SPH-containing ceramides (N-acylsphing-4-enines) as substrates, it also exhibits activity toward dihydrosphingosine-containing CERs (N-acylsphinganines) and produces 14Z-SPH-containing sphingolipids,which can be found in patients with DEGS1 mutations (PubMed : 37209771). Its desaturase mechanism involves an electron transfer facilitated by cytochrome b5 (PubMed : 37209771). FADS3 also acts as a methyl-end fatty acyl coenzyme A (CoA) desaturase that introduces a cis double bond between the preexisting double bond and the terminal methyl group of the fatty acyl chain (By similarity). Desaturates (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 13 to generate (11E,13Z)-octadecadienoate (also known as conjugated linoleic acid 11E,13Z-CLA) (By similarity).
See full target information FADS3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 10:218 PubMed35280359

2022

Panax notoginseng attenuates hypoxia-induced glycolysis in colonic mucosal epithelial cells in DSS-induced colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei He,Huafeng Pan,Ping Tao,Jiang Lin,Beiping Zhang,Shiying Wang
View all publications
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