AB202298

Anti-FAF1 antibody

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(2 Publications)

Rabbit Polyclonal FAF1 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Xenopus laevis, Mouse, Rat, Chicken, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAF1 aa 1-300.

View Alternative Names

UBXD12, UBXN3A, CGI-03, FAF1, FAS-associated factor 1, hFAF1, UBX domain-containing protein 12, UBX domain-containing protein 3A

5 Images

Flow Cytometry - Anti-FAF1 antibody (AB202298)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FAF1 antibody (AB202298)

Flow cytometric analysis of U2OS cells labelling FAF1 with ab202298 at 1/200 dilution. Labeling with IgG control (upper panel), ab202298 in Control siRNA-treated cells (middle panel) or ab202298 in FAF1 siRNA treated cells (lower panel) is shown. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.

Immunocytochemistry/ Immunofluorescence - Anti-FAF1 antibody (AB202298)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-FAF1 antibody (AB202298)

Immunofluorescent analysis of PFA fixed, methanol permeabilized U2OS cells transfected with HA-FAF1 labeling FAF1 with ab202298 at 1/600 dilution (red). HA (green).

Supplier Data

Immunoprecipitation - Anti-FAF1 antibody (AB202298)

Western blot analysis with ab202298 immunoprecipitation using U2OS cell extracts.

Lane 1 : Input.

Lane 2 : Immunoprecipitation with control serum.

Lane 3 : Immunoprecipitation with ab202298 (5 μL).

All lanes:

Immunoprecipitation - Anti-FAF1 antibody (ab202298)

Predicted band size: 74 kDa

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Supplier Data

Western blot - Anti-FAF1 antibody (AB202298)

All lanes:

Western blot - Anti-FAF1 antibody (ab202298) at 1/2000 dilution

Lane 1:

U2OS cell lysate transfected with Control siRNA at 40 µg

Lane 2:

U2OS cell lysate transfected with FAF1 siRNA at 40 µg

Lane 3:

NIH3T3 cell lysate at 40 µg

Lane 4:

INS1 cell lysate at 40 µg

Lane 5:

DT40 cell lysate at 40 µg

Predicted band size: 74 kDa

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Supplier Data

Western blot - Anti-FAF1 antibody (AB202298)

All lanes:

Western blot - Anti-FAF1 antibody (ab202298) at 1/2000 dilution

Lane 1:

Xenopus laevis crude egg extracts at 50 µg

Lane 2:

U2OS extracts at 50 µg

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Chicken, Human, Xenopus laevis

Applications

WB, ICC/IF, IP, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human FAF1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UNN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/600", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAF1 also known as FAS-associated factor 1 functions mechanically by interacting with the FAS receptor to modulate apoptotic processes. It has a molecular weight of approximately 74 kDa. This protein can be found in various tissues with higher expression in the brain liver and kidney. The expression suggests its diverse roles in cellular processes across different organ systems.

Biological function summary

FAF1 interacts with ubiquitin-like modifiers to influence the cellular response to stress. It is part of the death-inducing signaling complex (DISC) where it contributes to the regulation of apoptosis. In addition to apoptosis FAF1 plays a role in regulating protein degradation processes connecting its function to proteostasis in cells.

Pathways

FAF1 takes part in significant cellular processes including the apoptotic signaling pathway and the NF-kB signaling pathway. In these pathways it interacts with FAS and NF-kB proteins contributing to cell death and inflammatory responses respectively. The influence on these pathways showcases its role in maintaining balance between cell survival and cell death.

FAF1 displays connections to neurodegenerative diseases and cancers. It has been implicated in the pathogenesis of Parkinson's disease where its dysregulation can affect neuronal survival. Furthermore its interaction with FAS in the apoptosis pathway links FAF1 to certain types of cancers where apoptotic failure results in uncontrolled cell growth. Understanding FAF1's role is essential for developing therapeutic strategies targeting these pathways and associated conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-binding protein (PubMed : 19722279). Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed : 26842564). Potentiates but cannot initiate FAS-induced apoptosis (By similarity).

See full target information FAF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23770 PubMed40610502

2025

Discovering molecular signatures in kidney transplant biopsies with borderline changes and isolated V-lesions: single-cell RNA-sequencing analysis of human blood and tissue Spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongin Song,Minji Kang,Yunyoung Jang,Christine Suh-Yun Joh,Ha Yeon Shin,Julia Young Baik,Hyun Seung Choi,Kyu Hong Kim,Seong Min Lee,Seung-Min Cha,Hyunah Ku,Ahram Han,Sang Il Min,Jongwon Ha,Kyung Chul Moon,Sehoon Park,Seung Seok Han,Hajeong Lee,Dong Ki Kim,Kook Hwan Oh,Kwon Wook Joo,Ji Hwan Moon,Chung-Gyu Park,Yon Su Kim,Seung Hee Yang,Hyun Je Kim,Yong Chul Kim

Cell reports 42:112125 PubMed36807144

2023

TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away.

Applications

Species

Xenopus laevis

Tycho E T Mevissen,Anisa V Prasad,Johannes C Walter

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAF1 antibody

Flow Cytometry - Anti-FAF1 antibody (AB202298)

Immunocytochemistry/ Immunofluorescence - Anti-FAF1 antibody (AB202298)

Immunoprecipitation - Anti-FAF1 antibody (AB202298)

Western blot - Anti-FAF1 antibody (AB202298)

Western blot - Anti-FAF1 antibody (AB202298)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise