AB225556

Anti-FAF1 antibody

Name: Anti-FAF1 antibody (ab225556) | Abcam
Brand: Abcam Limited
SKU: AB225556
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal FAF1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FAF1 aa 1-100.

View Alternative Names

UBXD12, UBXN3A, CGI-03, FAF1, FAS-associated factor 1, hFAF1, UBX domain-containing protein 12, UBX domain-containing protein 3A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAF1 antibody (AB225556)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAF1 antibody (AB225556)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for FAF1 using ab225556 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAF1 antibody (AB225556)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for FAF1 using ab225556 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-FAF1 antibody (AB225556)

FAF1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225556 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225556 at 1 μg/ml.

Lane 1 : ab225556 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-FAF1 antibody (ab225556)

Predicted band size: 74 kDa

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Supplier Data

Western blot - Anti-FAF1 antibody (AB225556)

All lanes:

Western blot - Anti-FAF1 antibody (ab225556) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 74 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human FAF1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UNN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab225556 was affinity purified using an epitope specific to FAF1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAF1 also known as FAS-associated factor 1 functions mechanically by interacting with the FAS receptor to modulate apoptotic processes. It has a molecular weight of approximately 74 kDa. This protein can be found in various tissues with higher expression in the brain liver and kidney. The expression suggests its diverse roles in cellular processes across different organ systems.

Biological function summary

FAF1 interacts with ubiquitin-like modifiers to influence the cellular response to stress. It is part of the death-inducing signaling complex (DISC) where it contributes to the regulation of apoptosis. In addition to apoptosis FAF1 plays a role in regulating protein degradation processes connecting its function to proteostasis in cells.

Pathways

FAF1 takes part in significant cellular processes including the apoptotic signaling pathway and the NF-kB signaling pathway. In these pathways it interacts with FAS and NF-kB proteins contributing to cell death and inflammatory responses respectively. The influence on these pathways showcases its role in maintaining balance between cell survival and cell death.

FAF1 displays connections to neurodegenerative diseases and cancers. It has been implicated in the pathogenesis of Parkinson's disease where its dysregulation can affect neuronal survival. Furthermore its interaction with FAS in the apoptosis pathway links FAF1 to certain types of cancers where apoptotic failure results in uncontrolled cell growth. Understanding FAF1's role is essential for developing therapeutic strategies targeting these pathways and associated conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Ubiquitin-binding protein (PubMed : 19722279). Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed : 26842564). Potentiates but cannot initiate FAS-induced apoptosis (By similarity).

See full target information FAF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 26: PubMed36052865

2022

miR‑363‑3p attenuates the oxygen‑glucose deprivation/reoxygenation‑induced neuronal injury by targeting PDCD6IP.

Applications

Unspecified application

Species

Unspecified reactive species

Yihan Wang,Jiahui Jin,Zongxin Xia,Huisheng Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAF1 antibody (AB225556)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAF1 antibody (AB225556)

Immunoprecipitation - Anti-FAF1 antibody (AB225556)

Western blot - Anti-FAF1 antibody (AB225556)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise