AB40794

Anti-FAK antibody [EP695Y]

Name: Anti-FAK antibody [EP695Y] (ab40794) | Abcam
Brand: Abcam Limited
SKU: AB40794
Price: 240 USD
Availability: InStock
Rating: 5 (9 reviews)

20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

(9 Reviews)

(190 Publications)

Anti-FAK antibody [EP695Y] (ab40794) is a rabbit monoclonal antibody detecting FAK in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 140 publications
- Trusted since 2006

View Alternative Names

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1 : 250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

AbReview19322****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

Supplier Data

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of phosphorylated FAK is upregulated in response to pervanadate treatment (PMID : 20847951).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-FAK antibody [EP695Y] (ab40794) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] (<a href='/en-us/products/primary-antibodies/fak-phospho-y397-y576-y925-antibody-rm1141-ab322354'>ab322354</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

HeLa treated with 10μM pervanadate for 60 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated HeLa whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

HeLa treated with 10μM pervanadate for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 119 kDa,15 kDa

false

Exposure time: 8s

Supplier Data

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab322920 was shown to bind specifically to FAK (phospho S732). Target of interest was observed at 75-120 kDa in wild-type HEK-293 cell lysates (lane 2) with no signal observed at this size in FAK knockout cell line (lanes 3-4) (lane 3, knockout cell line ab255421 / knockout cell lysate ab263766).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35787638).

The identity of the higher MW band at approximately 250 kDa (in lanes 1-4) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-FAK antibody - Total protein control (ab40794) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (<a href='/en-us/products/primary-antibodies/fak-phospho-s732-antibody-epr26074-44-ab322920'>ab322920</a>) at 1/1000 dilution

Lane 1:

Untreated Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

Wild-type HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated FAK knockout HEK-293 whole cell lysate (untreated membrane) at 20 µg

Lane 4:

FAK knockout HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 5:

Untreated Wild-type HEK-293 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 6:

Wild-type HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 7:

Untreated FAK knockout HEK-293 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 8:

FAK knockout HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-120 kDa,36 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

All lanes:

Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Lab

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

** Lanes 1 - 4 : ** Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa. ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 2:

Western blot - Human PTK2 (FAK) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ptk2-fak-knockout-hek-293t-cell-line-ab255421'>ab255421</a>)

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

PTK2 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa,37 kDa

false

Supplier Data

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35787638).

The identity of the higher MW band at approximately 250 kDa (in lanes 1-2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-FAK antibody - Total protein control (ab40794) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (<a href='/en-us/products/primary-antibodies/fak-phospho-s732-antibody-epr26074-44-ab322920'>ab322920</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

NIH/3T3 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated NIH/3T3 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

NIH/3T3 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 59s

AbReview58760****

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot analysis of RAW264.7 cells lysate (40μg/lane) labelling FAK with ab40794 at 1/5000 in 5% Milk PBS Tween for 16 hours at 4°C. A HRP conjugated goat anti-rabbit poly clonal (1/5000) was used as the secondary antibody.

All lanes:

Western blot - Anti-FAK antibody [EP695Y] (ab40794)

Secondary

All lanes:

HRP conjugated goat anti-rabbit poly clonal at 1/5000 dilution

Predicted band size: 119 kDa

Observed band size: 130 kDa

true

Exposure time: 5min

This image is courtesy of an Abreview submitted by Magali Boissiere (6970246)

Supplier Data

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35787638).

The identity of the higher MW band at approximately 250 kDa (in lanes 1-2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-FAK antibody - Total protein control (ab40794) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (<a href='/en-us/products/primary-antibodies/fak-phospho-s732-antibody-epr26074-44-ab322920'>ab322920</a>) at 1/1000 dilution

Lane 1:

Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

PC-12 treated with 100nM Calycin A for 10 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated PC-12 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

PC-12 treated with 100nM Calycin A for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 15s

Supplier Data

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of phosphorylated FAK is upregulated in response to pervanadate treatment (PMID : 20847951).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-FAK antibody [EP695Y] (ab40794) staining at 1/1000 dilution.

All lanes:

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

NIH/3T3 treated with 10μM pervanadate for 60 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated NIH/3T3 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

NIH/3T3 treated with 10μM pervanadate for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 119 kDa,15 kDa

false

Exposure time: 8s

Supplier Data

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The activation of phosphorylation of FAK is reported to be related to developmental processes in brain tissue (PMID : 14642275). So expression level of phosphorylated modified FAK in normal brain is quite low causing not easy to be detected. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher-sensitivity ECL substrate) to improve results.

FAK can be cleaved at several sites. The molecular weight observed is consistent with what has been described in the literature (PMID : 10545505, PMID : 9642276).

This blot of Lane 2 and 4 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-FAK antibody [EP695Y] (ab40794) staining at 1/1000 dilution.

Exposure time : Lane 1, 3 : 136 seconds; Lane 2, 4 : 70 seconds

All lanes:

Lane 1:

Mouse brain tissue lysate (untreated membrane) at 40 µg

Lane 2:

Rat brain tissue lysate (untreated membrane) at 40 µg

Lane 3:

Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 40 µg

Lane 4:

Rat brain tissue lysate (alkaline phosphatase treated membrane) at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 119 kDa,36 kDa

false

Lab

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Lane 1:

Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution

Lane 2:

Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 2:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Unknown

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

All lanes:

Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

All lanes:

Hela cell lysate

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Carrier free

Anti-FAK antibody [EP695Y] - BSA and Azide free
Carrier free

Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-FAK antibody [EP695Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-FAK antibody [EP695Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-FAK antibody [EP695Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-FAK antibody [EP695Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-FAK antibody [EP695Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-FAK antibody [EP695Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP695Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab40794 recognises Focal adhesion kinase (FAK).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/1000", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/1000", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/1000", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-FAK antibody [EP695Y] (ab40794) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of FAK?
Anti-FAK [EP695Y] (ab40794) specifically detects a band for FAK (UniProt: Q05397) at a molecular weight of 119kDa.

Trusted by the scientific community
Anti-FAK [EP695Y] (ab40794) was first used in a scientific publication in 2006 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-FAK [EP695Y] (ab40794) has over 5 independent reviews from customers.

Specificity confirmed
The specificity of Anti-FAK antibody [EP695Y] (ab40794) has been confirmed by Western blot testing in PTK2 Knockout HEK-293 cells.

Other related products
We have a range of other formats of antibody clone [EP695Y] also available for your convenience: ab40794, Carrier free - ab219363, Carrier free - ab271836, Alexa Fluor^® 488 - ab311051, Alexa Fluor^® 647 - ab311177, Alexa Fluor^® 594 - ab311832, Alexa Fluor^® 568 - ab313115, Alexa Fluor^® 555 - ab313308, Alexa Fluor^® 750 - ab321621

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.

Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

See full target information PTK2

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Publications (190)

Recent publications for all applications. Explore the full list and refine your search

Radiology and oncology 59:349-367 PubMed40959921

2025

The role of focal adhesion kinase in bladder cancer: translation from to human urothelial carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Gaja Markovic,Natasa Resnik,Aleksandar Janev,Dasa Zupancic,Gasper Grubelnik,Marusa Debeljak,Maja Cemazar,Tanja Jesenko,Masa Omerzel,Tomaz Smrkolj,Mateja Erdani Kreft

Journal of gynecologic oncology : PubMed40784723

2025

FUS-stabilized USP7 facilitates the bevacizumab resistance of ovarian cancer through deubiquitinating PTK2.

Applications

Unspecified application

Species

Unspecified reactive species

Xianping Wen,Ruocheng Xu,Ranran Li,Shuo Li,Guantai Ni

Diabetology & metabolic syndrome 17:279 PubMed40682113

2025

Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Anjun Tan,Tianrong Li,Jingjing Yang,Xiaolu Li,Wenqin Li,Jinwen Yu

iScience 28:112176 PubMed40224016

2025

Injured tubular epithelia-derived CCN1 promotes the mobilization of fibroblasts toward injury sites after kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohiro Nakata,Yuhei Kirita,Minato Umehara,Masashi Nakamura,Shinji Sawai,Atsushi Minamida,Hiroko Yamauchi-Sawada,Yasuto Sunahara,Yayoi Matoba,Natsuko Okuno-Ozeki,Itaru Nakamura,Kunihiro Nakai,Aya Yagi-Tomita,Noriyuki Yamashita,Keiichi Tamagaki,Benjamin D Humphreys,Satoaki Matoba,Tetsuro Kusaba

Nature communications 16:2419 PubMed40075063

2025

Uptake of small extracellular vesicles by recipient cells is facilitated by paracrine adhesion signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Koichiro M Hirosawa,Yusuke Sato,Rinshi S Kasai,Eriko Yamaguchi,Naoko Komura,Hiromune Ando,Ayuko Hoshino,Yasunari Yokota,Kenichi G N Suzuki

Research (Washington, D.C.) 8:0619 PubMed39975575

2025

Regulating Integrin β1 to Restore Gonadotropin-Releasing Hormone-Tanycyte Unit Function in Polycystic Ovary Syndrome-Related Hypothalamic Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xiaoyu Tong,Yan Xiao,Yicong Wang,Wei Hu,Wenhan Lu,Yuning Chen,Jiajia Li,Wenhao Gao,Hongru Gao,Yicheng Tian,Sizhe Dai,Yi Feng

Chinese medicine 20:18 PubMed39910658

2025

Distinct mechanisms of electroacupuncture and manual acupuncture in modulating hypothalamic GnRH-tanycyte unit function of polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Yicong Wang,Yuning Chen,Wenhan Lu,Xiaoyu Tong,Jiajia Li,Wenhao Gao,Rui Huang,Wei Hu,Yi Feng

World journal of gastroenterology 31:100898 PubMed39811500

2025

promotes the progression of gastric cancer through the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Yu Chen,Pei-Yao Wang,Bang Liu,Yang-Pu Jia,Zhao-Xiong Zhang,Xin Liu,Dao-Han Wang,Yong-Jia Yan,Wei-Hua Fu,Feng Zhu

The Journal of biological chemistry 301:108155 PubMed39761856

2025

Unveiling ADAMTS12: A key driver of bladder cancer progression via COL3A1-Mediated activation of the FAK/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Hua Xiao,Li-Zhe Xu,Jin-Zhuo Ning,Fan Cheng

Hepatology communications 8: PubMed39761008

2025

FAK inhibition delays liver repair after acetaminophen-induced acute liver injury by suppressing hepatocyte proliferation and macrophage recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Qi Xu,Jialin Shi,Wei Dong,Junfei Jin,Chong Zhang

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Anti-FAK antibody [EP695Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Western blot - Anti-FAK antibody [EP695Y] (AB40794)

Related conjugates and formulations (8)

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Publications (190)

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