Rabbit Recombinant Monoclonal FAK antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 146 publications.
Images
![Western blot - Anti-FAK antibody [EP695Y] (AB40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img187.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (AB40794)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27986.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)")
![Western blot - Anti-FAK antibody [EP695Y] (AB40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img156895.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (AB40794)")
![Western blot - Anti-FAK antibody [EP695Y] (AB40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img64488.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (AB40794)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133416.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)")
Publications
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- Biological research 56:662023The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Chia Wu,Wei-Ting Wang,Ming-Chun Yang,Yu-Tsun Su,Jwu-Lai Yeh,Jong-Hau Hsu,Jiunn-Ren WuPubMed 38057829
- Journal of inflammation research 16:5095-51092023LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi ChenPubMed 38026263
- Journal of materials science. Materials in medicine 34:542023Unusual effects of a nanoporous gold substrate on cell adhesion and differentiation because of independent multi-branch signaling of focal adhesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeizheng Wu,Kazuya Yanagi,Kazuki Yokota,Masataka Hakamada,Mamoru MabuchiPubMed 37884819
- International immunopharmacology 124:1109482023PP2 alleviates the progression of osteoarthritis by inhibiting Wnt/β-catenin and activating TGF-β/Smad signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhang,Chuanchuan Zheng,Wei Chen,Xiaoqiang Li,Jinshu Wang,Taikun Wang,Qi Zhao,Hao Huang,Yiting Li,Chengliang Yang,Kegong Xie,Shengcai Pan,Binghao Wang,Chong Wang,Yujin Tang,Kai Li,Jia Liu,Liqiang WangPubMed 37774483
- Cell death discovery 9:3242023N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBen Wang,Jin Zhang,Guo Li,Chenzhong Xu,Langmei Yang,Jie Zhang,Yalan Wu,Ye Liu,Zuojun Liu,Ming Wang,Ji Li,Xiaolong Tang,Baohua LiuPubMed 37644005
- Frontiers in physiology 14:12189242023The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCédric H G Neutel,Callan D Wesley,Guido R Y De Meyer,Wim Martinet,Pieter-Jan GunsPubMed 37637147
- Journal of orthopaedic translation 40:58-712023Osteocyte β3 integrin promotes bone mass accrual and force-induced bone formation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Qin,Zecai Chen,Dazhi Yang,Tailin He,Zhen Xu,Peijun Zhang,Di Chen,Weihong Yi,Guozhi XiaoPubMed 37457310
- Chinese medicine 18:342023Psoralidin inhibits osteosarcoma growth and metastasis by downregulating ITGB1 expression via the FAK and PI3K/Akt signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengwen Cheng,Senrui Liu,Bowen Chen,Chengcheng Du,Pengcheng Xiao,Xuefeng Luo,Li Wei,Yiting Lei,Chen Zhao,Wei HuangPubMed 37004120
- Frontiers in pharmacology 13:11035572023schischkin and bunge promote angiogenesis to treat myocardial ischemia Ang-1/Tie-2/FAK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMu-Xin Zhang,Xue-Ying Huang,Yu Song,Wan-Li Xu,Yun-Lun Li,Chao LiPubMed 36699092
- Frontiers in molecular biosciences 9:9845642022Identification of PTK2 as an adverse prognostic biomarker in breast cancer by integrated bioinformatics and experimental analyses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanru Chen,Wei Wang,Lingyu Fang,Zhenyang Zhang,Shishan DengPubMed 36533074
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes For unpurified use at 1/1000 |
Species Human | Dilution info 1/2000 | Notes For unpurified use at 1/1000 |
Species Rat | Dilution info 1/2000 | Notes For unpurified use at 1/1000 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed:9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.Isoform 6Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Alternative names
FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71
Recommended products
Rabbit Recombinant Monoclonal FAK antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 146 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP695Y
- Purification technique
Affinity purification Protein A
- Specificity
ab40794 recognises Focal adhesion kinase (FAK).
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.
- Biological function summary
Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.
- Pathways
Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.
- Associated diseases and disorders
Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img187.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
** Lanes 1 - 4:** Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human PTK2 (FAK) knockout HEK-293T cell line ab255421 (knockout cell lysate Human PTK2 (FAK) knockout HEK-293T cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: Wild-type HEK-293T cell lysate at 20 µg
Lane 4: PTK2 knockout HEK-293T cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 119 kDa, 37 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human PTK2 (FAK) knockout HEK-293T cell line ab255421 (knockout cell lysate Human PTK2 (FAK) knockout HEK-293T cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27986.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img156895.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
All lanes: Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 119 kDa
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img64488.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Lane 1: Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution
Lane 2: Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 2: Rat brain lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 119 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133416.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img94722.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
All lanes: Western blot - Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution
All lanes: Hela cell lysate
Predicted band size: 119 kDa
Observed band size: 119 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140241.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)")
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)
The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells.
The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img6463.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
This image is courtesy of a customer review submitted by Magali Boissiere (6970246)Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Western blot analysis of RAW264.7 cells lysate (40μg/lane) labelling FAK with ab40794 at 1/5000 in 5% Milk PBS Tween for 16 hours at 4°C. A HRP conjugated goat anti-rabbit poly clonal (1/5000) was used as the secondary antibody.
All lanes: Western blot - Anti-FAK antibody [EP695Y] (ab40794)
SecondaryAll lanes: HRP conjugated goat anti-rabbit poly clonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 130 kDa
Exposure time: 5min
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img440980.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-FAK (phospho S732) antibody [EPR26074-44] ab322920 was shown to bind specifically to FAK (phospho S732). Target of interest was observed at 75-120 kDa in wild-type HEK-293 cell lysates (lane 2) with no signal observed at this size in FAK knockout cell line (lanes 3-4) (lane 3, knockout cell line Human PTK2 (FAK) knockout HEK-293T cell line ab255421 / knockout cell lysate Human PTK2 (FAK) knockout HEK-293T cell lysate ab263766).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35787638).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-4) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody - Total protein control (ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (Anti-FAK (phospho S732) antibody [EPR26074-44] ab322920) at 1/1000 dilution
Lane 1: Untreated Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: Wild-type HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated FAK knockout HEK-293 whole cell lysate (untreated membrane) at 20 µg
Lane 4: FAK knockout HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 5: Untreated Wild-type HEK-293 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 6: Wild-type HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 7: Untreated FAK knockout HEK-293 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 8: FAK knockout HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 75-120 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img438692.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of phosphorylated FAK is upregulated in response to pervanadate treatment (PMID: 20847951).
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-FAK antibody [EP695Y] (ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] (Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] ab322354) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg
Lane 2: NIH/3T3 treated with 10μM pervanadate for 60 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated NIH/3T3 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: NIH/3T3 treated with 10μM pervanadate for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 119 kDa, 15 kDa
Exposure time: 8s
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img438691.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of phosphorylated FAK is upregulated in response to pervanadate treatment (PMID: 20847951).
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-FAK antibody [EP695Y] (ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] (Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] ab322354) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: HeLa treated with 10μM pervanadate for 60 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated HeLa whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: HeLa treated with 10μM pervanadate for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 119 kDa, 15 kDa
Exposure time: 8s
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img440982.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35787638).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody - Total protein control (ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (Anti-FAK (phospho S732) antibody [EPR26074-44] ab322920) at 1/1000 dilution
Lane 1: Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: PC-12 treated with 100nM Calycin A for 10 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated PC-12 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: PC-12 treated with 100nM Calycin A for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 15s
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img440981.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35787638).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody - Total protein control (ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (Anti-FAK (phospho S732) antibody [EPR26074-44] ab322920) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg
Lane 2: NIH/3T3 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated NIH/3T3 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: NIH/3T3 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 59s
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img438693.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Western blot - Anti-FAK antibody [EP695Y] (ab40794)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The activation of phosphorylation of FAK is reported to be related to developmental processes in brain tissue (PMID: 14642275). So expression level of phosphorylated modified FAK in normal brain is quite low causing not easy to be detected. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher-sensitivity ECL substrate) to improve results.
FAK can be cleaved at several sites. The molecular weight observed is consistent with what has been described in the literature (PMID: 10545505, PMID: 9642276).
This blot of Lane 2 and 4 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody [EP695Y] (ab40794) staining at 1/1000 dilution.
Exposure time: Lane 1, 3: 136 seconds; Lane 2, 4: 70 seconds
All lanes: Western blot - Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] (Anti-FAK (phospho Y397 + Y576 + Y925) antibody [RM1141] ab322354) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate (untreated membrane) at 40 µg
Lane 2: Rat brain tissue lysate (untreated membrane) at 40 µg
Lane 3: Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 40 µg
Lane 4: Rat brain tissue lysate (alkaline phosphatase treated membrane) at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 119 kDa, 36 kDa
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img94722.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140241.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img6463.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img440980.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img438692.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img438691.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img440982.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img440981.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
![Western blot - Anti-FAK antibody [EP695Y] (ab40794), expandable thumbnail](https://content.abcam.com/products/images/fak-antibody-ep695y-ab40794--western-blot-img438693.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
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