AB219363

Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free

RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(27 Publications)

Rabbit Recombinant Monoclonal FAK antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 27 publications.

View Alternative Names

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1 : 250 dilution (2.32 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).

IHC-P

AbReview19322****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).

This image is courtesy of an Abreview submitted by Carl Hobbs.

Lab

Western blot - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

This data was developed using the same antibody clone in a different buffer formulation (ab40794).

Lanes 1 - 4 : Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FAK antibody [EP695Y] (<a href='/en-us/products/primary-antibodies/fak-antibody-ep695y-ab40794'>ab40794</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 2:

Western blot - Human PTK2 (FAK) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ptk2-fak-knockout-hek-293t-cell-line-ab255421'>ab255421</a>)

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

PTK2 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa,37 kDa

false

Carrier free

Anti-FAK antibody [EP695Y] - BSA and Azide free
Unconjugated

Anti-FAK antibody [EP695Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-FAK antibody [EP695Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-FAK antibody [EP695Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-FAK antibody [EP695Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-FAK antibody [EP695Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-FAK antibody [EP695Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-FAK antibody [EP695Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP695Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab219363 recognises Focal adhesion kinase (FAK).

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For unpurified use at 1/1000", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For unpurified use at 1/1000", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab219363 is the carrier-free version of ab40794.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.

Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

See full target information PTK2

style

left-column

Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Oral diseases 30:562-574 PubMed36519511

2022

Substrate stiffness promotes dentinogenesis via LAMB1-FAK-MEK1/2 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingru Bai,Huiyu Chen,Zhaowei Zhang,Xiaoyu Liu,Demao Zhang,Chengling Wang

International journal of oral science 14:15 PubMed35277477

2022

Microenvironmental stiffness mediates cytoskeleton re-organization in chondrocytes through laminin-FAK mechanotransduction.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Zhou,Mengmeng Duan,Daimo Guo,Xinmei Du,Demao Zhang,Jing Xie

Cell proliferation 55:e13172 PubMed34953003

2021

Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxuan Wang,Jing Xie,Chenchen Zhou,Wenli Lai

ACS applied materials & interfaces 11:26448-26459 PubMed31251564

2019

Substrate Compliance Directs the Osteogenic Lineages of Stem Cells from the Human Apical Papilla via the Processes of Mechanosensing and Mechanotransduction.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Zhou,Demao Zhang,Jing Zou,Xiaobing Li,Shujuan Zou,Jing Xie

Journal of cell science 129:743-56 PubMed26769900

2016

Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase.

Applications

IP, IHC-Fr

Species

Human, Human

Jui M Dave,Colette A Abbey,Camille L Duran,Heewon Seo,Gregory A Johnson,Kayla J Bayless

Nature 527:329-35 PubMed26524530

2015

Tumour exosome integrins determine organotropic metastasis.

Applications

Unspecified application

Species

Human

Ayuko Hoshino,Bruno Costa-Silva,Tang-Long Shen,Goncalo Rodrigues,Ayako Hashimoto,Milica Tesic Mark,Henrik Molina,Shinji Kohsaka,Angela Di Giannatale,Sophia Ceder,Swarnima Singh,Caitlin Williams,Nadine Soplop,Kunihiro Uryu,Lindsay Pharmer,Tari King,Linda Bojmar,Alexander E Davies,Yonathan Ararso,Tuo Zhang,Haiying Zhang,Jonathan Hernandez,Joshua M Weiss,Vanessa D Dumont-Cole,Kimberly Kramer,Leonard H Wexler,Aru Narendran,Gary K Schwartz,John H Healey,Per Sandstrom,Knut Jørgen Labori,Elin H Kure,Paul M Grandgenett,Michael A Hollingsworth,Maria de Sousa,Sukhwinder Kaur,Maneesh Jain,Kavita Mallya,Surinder K Batra,William R Jarnagin,Mary S Brady,Oystein Fodstad,Volkmar Muller,Klaus Pantel,Andy J Minn,Mina J Bissell,Benjamin A Garcia,Yibin Kang,Vinagolu K Rajasekhar,Cyrus M Ghajar,Irina Matei,Hector Peinado,Jacqueline Bromberg,David Lyden

Biophysical journal 109:57-65 PubMed26153702

2015

The cytoskeleton regulates cell attachment strength.

Applications

Species

Unspecified reactive species

Alexander Fuhrmann,Adam J Engler

BMC cancer 15:266 PubMed25886367

2015

The involvement of insulin-like growth factor 2 binding protein 3 (IMP3) in pancreatic cancer cell migration, invasion, and adhesion.

Applications

Species

Human

Clarissa C Pasiliao,Che-Wei A Chang,Brent W Sutherland,Shannon M Valdez,David Schaeffer,Donald T Yapp,Sylvia S W Ng

PloS one 10:e0116270 PubMed25643152

2015

Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ∆133p53α mimic.

Applications

Species

Mouse

Sonal Sawhney,Kylie Hood,Alisha Shaw,Antony W Braithwaite,Richard Stubbs,Noelyn A Hung,Janice A Royds,Tania L Slatter

Molecular cancer research : MCR 13:223-30 PubMed25582703

2015

A mechanism for asymmetric cell division resulting in proliferative asynchronicity.

Applications

IP, WB

Species

Human, Human

Ipsita Dey-Guha,Cleidson P Alves,Albert C Yeh, Salony,Xavier Sole,Revati Darp,Sridhar Ramaswamy

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

Western blot - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (AB219363)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

Product promise