Rabbit Recombinant Monoclonal FAK phospho S732 antibody. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
Dot | WB | ICC/IF | IHC-P | |
---|---|---|---|---|
Human | Expected | Tested | Not recommended | Not recommended |
Mouse | Expected | Tested | Not recommended | Not recommended |
Rat | Expected | Tested | Not recommended | Not recommended |
Synthetic peptide - Human | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed:9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.Isoform 6Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
FAK, FAK1, FAK, PTK2, FAK1, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71
Rabbit Recombinant Monoclonal FAK phospho S732 antibody. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR26074-44
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Dot blot analysis of FAK (phospho S732) using ab322920 at 1000 (0.531 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: FAK (phospho S732) peptide a
Lane2: FAK (phospho S732) peptide b
Lane3: FAK non-phospho peptide c
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (ab322920) at 1/1000 dilution
Lane 1: FAK (phospho S732) peptide a
Lane 2: FAK (phospho S732) peptide b
Lane 3: FAK non-phospho peptide c
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, ab322920 was shown to bind specifically to FAK (phospho S732). Target of interest was observed at 75-120 kDa in wild-type HEK-293 cell lysates (lane 2) with no signal observed at this size in FAK knockout cell line (lanes 3-4) (lane 3, knockout cell line Human PTK2 (FAK) knockout HEK-293T cell line ab255421 / knockout cell lysate Human PTK2 (FAK) knockout HEK-293T cell lysate ab263766).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35787638).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-4) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody - Total protein control (Anti-FAK antibody [EP695Y] ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (ab322920) at 1/1000 dilution
Lane 1: Untreated Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: Wild-type HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated FAK knockout HEK-293 whole cell lysate (untreated membrane) at 20 µg
Lane 4: FAK knockout HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 5: Untreated Wild-type HEK-293 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 6: Wild-type HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 7: Untreated FAK knockout HEK-293 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 8: FAK knockout HEK-293 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 75-120 kDa, 36 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35787638).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody - Total protein control (Anti-FAK antibody [EP695Y] ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (ab322920) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg
Lane 2: NIH/3T3 treated with 100nM Calycin A for 30 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated NIH/3T3 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: NIH/3T3 treated with 100nM Calycin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 59s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35787638).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-FAK antibody - Total protein control (Anti-FAK antibody [EP695Y] ab40794) staining at 1/1000 dilution.
All lanes: Western blot - Anti-FAK (phospho S732) antibody [EPR26074-44] (ab322920) at 1/1000 dilution
Lane 1: Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg
Lane 2: PC-12 treated with 100nM Calycin A for 10 minutes whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated PC-12 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: PC-12 treated with 100nM Calycin A for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 15s
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