AB81298

Anti-FAK (phospho Y397) antibody [EP2160Y]

Name: Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) | Abcam
Brand: Abcam Limited
SKU: AB81298
Price: 240 USD
Availability: InStock
Rating: 4 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(185 Publications)

Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) is a rabbit monoclonal antibody detecting FAK in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 130 publications
- Trusted since 2009

View Alternative Names

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

4 Images

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Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

All lanes:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/2000 dilution

Lane 1:

human brain tissue lysates, untreated at 10 µg

Lane 2:

human brain tissue lysates treated with AP at 10 µg

Lane 3:

rat brain tissue lysates, untreated at 10 µg

Lane 4:

rat brain tissue lysates treated with AP at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

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Supplier Data

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. The activation of phosphorylation of FAK is reported to be related to developmental processes in brain tissue (PMID : 14642275, PMID : 21118706). So expression level of phosphorylated modified FAK in normal brain is quite low causing not easy to be detected. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results. ab181602 has been used as a loading control.

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lanes 1 - 5:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/1000 dilution

Lane 2:

NIH/3T3 treated with 10mM Pervanadate for 60 min whole cell lysate at 20 µg

Lane 3:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa treated with 10mM Pervanadate for 60 min whole cell lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Exposure time: 40s

Unknown

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/2000 dilution

Lane 1:

Untreated mouse brain at 10 µg

Lane 2:

Mouse brain treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Unknown

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/1000 dilution

Lane 1:

Untreated rat brain at 10 µg

Lane 2:

Rat brain treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1000 µg

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Carrier free

Anti-FAK (phospho Y397) antibody [EP2160Y] - Low endotoxin, Azide free
Carrier free

Anti-FAK (phospho Y397) antibody [EP2160Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2160Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The activation of phosphorylation of FAK is reported to be related to developmental processes in brain tissue (PMID: 14642275, PMID: 21118706). So expression level of phosphorylated modified FAK in normal brain is quite low causing not easy to be detected. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of FAK?
Anti-FAK (phospho Y397) [EP2160Y] (ab81298) specifically detects a band for FAK (UniProt: Q05397) at a molecular weight of 119kDa.

Trusted by the scientific community
Anti-FAK (phospho Y397) [EP2160Y] (ab81298) was first used in a scientific publication in 2009 and has been cited over 130 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP2160Y] also available for your convenience: ab81298, Carrier free - ab223529, Carrier free - ab271862

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.

Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

See full target information PTK2 pY397

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Publications (185)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed41002379

2025

Mechanotransduction-Mediated Expansion of Rabbit Vocal Fold Epithelial Cells via ROCK Inhibition and Stromal Cell-Derived Paracrine Signals.

Applications

Unspecified application

Species

Unspecified reactive species

Samjhana Thapa,Joo Hyun Kim,Jun Yeong Jeong,Sung Sik Hur,Seung Won Lee,Yongsung Hwang

Animals : an open access journal from MDPI 15: PubMed40867704

2025

Activation of Focal Adhesion Pathway by as Key Regulatory Axis in Lipid Deposition in Goat Intramuscular Precursor Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Shao,Qi Li,Yu Liao,Yong Wang,Yaqiu Lin,Hua Xiang,Zhanyu Du,Changhui Zhang,Jiangjiang Zhu,Lian Huang

Soft matter 21:6779-6790 PubMed40813347

2025

Stabilizing light-responsive azobenzene films in an aqueous environment with thin polymer coatings.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Isomäki,Lotta Kääriäinen,Chiara Fedele,Suvi Lehtimäki,Tero-Petri Ruoko,Elina Mäntylä,Teemu O Ihalainen,Arri Priimagi

Discover oncology 16:1527 PubMed40788351

2025

Screening drugs of Anemoside B4 for MFAP4 expression in osteosarcoma in PDTX to increase personalized medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Lijuan Zeng,Jing Zhang,Zhiwei He,Xiaozhou Liu

Cell death discovery 11:216 PubMed40319030

2025

High Tau expression correlates with reduced invasion and prolonged survival in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Florencia Cidre-Aranaz,Claudia Magrin,Malenka Zimmermann,Jing Li,Annalisa Baffa,Matteo Ciccaldo,Wolfgang Hartmann,Uta Dirksen,Martina Sola,Paolo Paganetti,Thomas G P Grünewald,Stéphanie Papin

Communications biology 8:595 PubMed40240542

2025

Acoustic modulation of mechanosensitive genes and adipocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Kumeta,Makoto Otani,Masahiro Toyoda,Shige H Yoshimura

Communications biology 8:480 PubMed40164758

2025

Protein covariation networks for elucidating ferroptosis inducer mechanisms and potential synergistic drug targets.

Applications

Unspecified application

Species

Unspecified reactive species

Rina Kunishige,Yoshiyuki Noguchi,Naomi Okamoto,Lei Li,Akito Ono,Masayuki Murata,Fumi Kano

Research (Washington, D.C.) 8:0619 PubMed39975575

2025

Regulating Integrin β1 to Restore Gonadotropin-Releasing Hormone-Tanycyte Unit Function in Polycystic Ovary Syndrome-Related Hypothalamic Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xiaoyu Tong,Yan Xiao,Yicong Wang,Wei Hu,Wenhan Lu,Yuning Chen,Jiajia Li,Wenhao Gao,Hongru Gao,Yicheng Tian,Sizhe Dai,Yi Feng

Chinese medicine 20:18 PubMed39910658

2025

Distinct mechanisms of electroacupuncture and manual acupuncture in modulating hypothalamic GnRH-tanycyte unit function of polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Yicong Wang,Yuning Chen,Wenhan Lu,Xiaoyu Tong,Jiajia Li,Wenhao Gao,Rui Huang,Wei Hu,Yi Feng

iScience 28:111745 PubMed39898029

2025

Nuclear talin-1 provides a bridge between cell adhesion and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro J Da Silva,Hendrik S E Hästbacka,Mikael C Puustinen,Jenny C Pessa,Jens C Luoto,Erika Sundström,Benjamin T Goult,Guillaume Jacquemet,Eva Henriksson,Lea Sistonen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAK (phospho Y397) antibody [EP2160Y]

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (185)

Product promise