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AB226847

Anti-FAK (phospho Y576) antibody

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(2 Publications)

Rabbit Polyclonal FAK phospho Y576 antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment, Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PTK2 pY576.

View Alternative Names

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAK (phospho Y576) antibody (AB226847)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAK (phospho Y576) antibody (AB226847)

4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling FAK (phospho Y576) (green) using ab226847 at 1/500 dilution in ICC/IF. Nuclear Counterstain : Hoechst 33342.

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)
  • WB

Supplier Data

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody (ab226847) at 1/500 dilution

Lane 1:

Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with FAK shRNA whole cell extract at 50 µg

Predicted band size: 119 kDa

false

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)
  • WB

Supplier Data

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody (ab226847) at 1/3000 dilution

Lane 1:

Untreated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

EGF-treated (50 ng/ml for 30 min) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 3:

Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 4:

EGF-treated (200 ng/ml for 30 min) HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 119 kDa

false

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)
  • WB

Supplier Data

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody (ab226847) at 1/500 dilution

Lane 1:

HeLa whole cell extracts with starvation 24hr at 30 µg

Lane 2:

HeLa whole cell extracts with starvation 24hr, treated with 200ng/ml EGF, 15mins at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 119 kDa

false

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)
  • WB

Supplier Data

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody (ab226847) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

C8D30 whole cell extracts at 30 µg

Lane 3:

NIH-3T3 whole cell extracts at 30 µg

Lane 4:

Raw264.7 whole cell extracts at 30 µg

Lane 5:

C2c12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 119 kDa

false

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)
  • WB

Supplier Data

Western blot - Anti-FAK (phospho Y576) antibody (AB226847)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody (ab226847) at 1/500 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 119 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human PTK2 pY576. The exact immunogen used to generate this antibody is proprietary information.

Q05397

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.
Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
See full target information PTK2 pY576
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113034 PubMed40734678

2025

Preadipocyte-induced upregulation of IGFBP5 enhances ovarian cancer tumorigenesis via CREB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Waters,Cassidy Lucht,Sofia Howe,Gregory J Jordan,Ixchel Urbano,Samuel F Gilbert,Richard M Cripps,Mikella Robinson,Carrie D House

Nutrients 17: PubMed40573155

2025

Silkworm Enzyme Hydrolysates Improve Memory in MCI Models via CREB-BDNF Signaling and Enhanced Brain Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Yoo-Hee Kim,Nguyen Phuong,Nguyen Minh Anh Hoang,Hye-Jin Kim,Moo-Yeol Baik,Young Ho Koh
View all publications
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