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AB247442

Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal FAK phospho Y576 antibody. Carrier free. Suitable for Dot, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Mouse, Human samples.

View Alternative Names

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

5 Images
Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)
  • WB

Lab

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

This data was developed using ab76120, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (<a href='/en-us/products/primary-antibodies/fak-phospho-y576-antibody-ep1897y-ab76120'>ab76120</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate at 10 µg

Lane 3:

NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 125 kDa

false

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)
  • WB

Lab

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

This data was developed using ab76120, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (<a href='/en-us/products/primary-antibodies/fak-phospho-y576-antibody-ep1897y-ab76120'>ab76120</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate at 10 µg

Lane 3:

HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 125 kDa

false

Flow Cytometry (Intracellular) - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

This data was developed using ab76120, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells treated with 1mM Pervanadate for 30mins cells labeling FAK (phospho Y576)with ab76120 at 1/400 dilution (0.1μg) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730 / Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Dot Blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)
  • Dot

Unknown

Dot Blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

This data was developed using ab76120, the same antibody clone in a different buffer formulation.

Dot blot analysis of FAK (phospho Y576) phospho peptide (Lane 1) and FAK non-phospho peptide (Lane 2) labelling FAK (phospho Y576) phospho peptide with ab76120 at a dilution of 1/1000 dilution (0.441ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST .

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)
  • WB

Unknown

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

This data was developed using ab76120, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (<a href='/en-us/products/primary-antibodies/fak-phospho-y576-antibody-ep1897y-ab76120'>ab76120</a>) at 1/50000 dilution

Lane 1:

3T3 cell lysates untreated at 10 µg

Lane 2:

3T3 cell lysates treated with pervanadate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 119 kDa

Observed band size: 125 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1897Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247442 is the carrier-free version of ab76120.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.
Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
See full target information PTK2 phospho Y576
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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