Rabbit Polyclonal FAM111A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAM111A aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FAM111A aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PZ2
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Single-stranded DNA-binding serine protease that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed:32165630). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed:32165630). Protects replication fork from stalling by removing DPCs, such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesion, or poly(ADP-ribose) polymerase 1 (PARP1)-DNA complexes trapped by PARP inhibitors (PubMed:32165630). Required for PCNA loading on replication sites (PubMed:24561620). Promotes S-phase entry and DNA synthesis (PubMed:24561620). Acts also as a restriction factor for some viruses including SV40 polyomavirus and vaccinia virus (PubMed:23093934, PubMed:37607234). Mechanistically, affects nuclear barrier function during viral replication by mediating the disruption of the nuclear pore complex (NPC) via its protease activity (PubMed:33369867, PubMed:37607234). In turn, interacts with vaccinia virus DNA-binding protein OPG079 in the cytoplasm and promotes its degradation without the need of its protease activity but through autophagy (PubMed:37607234).
Alternative names
KIAA1895, FAM111A, Serine protease FAM111A
Recommended products
Rabbit Polyclonal FAM111A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAM111A aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FAM111A aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PZ2
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FAM111A also known as Family with Sequence Similarity 111 Member A is a protein with a molecular mass of approximately 66 kDa. It is expressed in various tissues including the spleen thymus and liver. FAM111A possesses a serine protease domain which links it to protease activity. Its expression is especially noted in actively proliferating cells suggesting a function in cell cycle regulation.
- Biological function summary
FAM111A participates in DNA replication and cell division. It often associates with components of the DNA replication machinery acting to maintain genomic stability during the cell cycle. Researchers have identified FAM111A as an essential factor for proper DNA synthesis. The protein may also interact with other cellular factors to ensure accurate DNA replication although it does not appear to form part of a large stable complex.
- Pathways
Studies connect FAM111A to the DNA replication and repair pathways. It interacts with proteins involved in maintaining the integrity of the genome such as those in the replication fork machinery. FAM111A participates alongside proteins like DNA polymerase and helicase where it contributes to their regulation and function. This involvement places FAM111A as an important component for effective DNA damage response and repair.
- Associated diseases and disorders
Mutations in FAM111A are linked to Kenny-Caffey Syndrome and Gracile Bone Dysplasia. These relate to abnormal growth and skeletal development. The protein's dysfunction disrupts normal cell cycle and growth regulator mechanisms. Alterations affecting FAM111A may also involve interactions with cyclins or cyclin-dependent kinases important for proper cell cycle progression. Understanding FAM111A's role can provide insights into therapeutic approaches for managing these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM111A antibody (ab234868)
Paraffin-embedded human liver cancer tissue stained for FAM111A using ab234868 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody (ab234868)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FAM111A (green) using ab234868 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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