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AB234868

Anti-FAM111A antibody

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(2 Publications)

Rabbit Polyclonal FAM111A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM111A aa 1-200.

View Alternative Names

KIAA1895, FAM111A, Serine protease FAM111A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM111A antibody (AB234868)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM111A antibody (AB234868)

Paraffin-embedded human liver cancer tissue stained for FAM111A using ab234868 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody (AB234868)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody (AB234868)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FAM111A (green) using ab234868 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FAM111A aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q96PZ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM111A also known as Family with Sequence Similarity 111 Member A is a protein with a molecular mass of approximately 66 kDa. It is expressed in various tissues including the spleen thymus and liver. FAM111A possesses a serine protease domain which links it to protease activity. Its expression is especially noted in actively proliferating cells suggesting a function in cell cycle regulation.
Biological function summary

FAM111A participates in DNA replication and cell division. It often associates with components of the DNA replication machinery acting to maintain genomic stability during the cell cycle. Researchers have identified FAM111A as an essential factor for proper DNA synthesis. The protein may also interact with other cellular factors to ensure accurate DNA replication although it does not appear to form part of a large stable complex.

Pathways

Studies connect FAM111A to the DNA replication and repair pathways. It interacts with proteins involved in maintaining the integrity of the genome such as those in the replication fork machinery. FAM111A participates alongside proteins like DNA polymerase and helicase where it contributes to their regulation and function. This involvement places FAM111A as an important component for effective DNA damage response and repair.

Mutations in FAM111A are linked to Kenny-Caffey Syndrome and Gracile Bone Dysplasia. These relate to abnormal growth and skeletal development. The protein's dysfunction disrupts normal cell cycle and growth regulator mechanisms. Alterations affecting FAM111A may also involve interactions with cyclins or cyclin-dependent kinases important for proper cell cycle progression. Understanding FAM111A's role can provide insights into therapeutic approaches for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-binding serine protease that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed : 32165630). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed : 32165630). Protects replication fork from stalling by removing DPCs, such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesion, or poly(ADP-ribose) polymerase 1 (PARP1)-DNA complexes trapped by PARP inhibitors (PubMed : 32165630). Required for PCNA loading on replication sites (PubMed : 24561620). Promotes S-phase entry and DNA synthesis (PubMed : 24561620). Also acts as a restriction factor for some viruses including SV40 polyomavirus and vaccinia virus (PubMed : 23093934, PubMed : 37607234). Mechanistically, affects nuclear barrier function during viral replication by mediating the disruption of the nuclear pore complex (NPC) via its protease activity (PubMed : 33369867, PubMed : 37607234). In turn, interacts with vaccinia virus DNA-binding protein OPG079 in the cytoplasm and promotes its degradation without the need of its protease activity but through autophagy (PubMed : 37607234).
See full target information FAM111A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 70:78-84 PubMed38836678

2024

M2 macrophages regulate KDM6B/PFKFB2 metabolic reprogramming of cervical squamous cell carcinoma through CXCL1.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Longzhang Huang,Lihua Cao

Aging 16:7578-7595 PubMed38568089

2024

Establishment and verification of a prognostic model based on coagulation and fibrinolysis-related genes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Fan,Le Lu,Hao Shang,Yuxuan Lu,Yi Yang,Xiuyan Wang,Hongwei Lu
View all publications
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Product promise

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