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AB184572

Anti-FAM111A antibody [EPR14407]

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(10 Publications)

Rabbit Recombinant Monoclonal FAM111A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications.

View Alternative Names

KIAA1895, FAM111A, Serine protease FAM111A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] (AB184572)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] (AB184572)

Immunofluorescent analysis of 4% paraformaldehyde-fixed T47-D cells labeling FAM111A with ab184572 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/1200 dilution (red). Counter stained with Dapi (blue).

Western blot - Anti-FAM111A antibody [EPR14407] (AB184572)
  • WB

Supplier Data

Western blot - Anti-FAM111A antibody [EPR14407] (AB184572)

All lanes:

Western blot - Anti-FAM111A antibody [EPR14407] (ab184572) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 20 µg

Lane 2:

BxPC-3 cell lysate at 20 µg

Lane 3:

T47-D cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

  • Carrier free

    Anti-FAM111A antibody [EPR14407] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14407

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM111A also known as Family with Sequence Similarity 111 Member A is a protein with a molecular mass of approximately 66 kDa. It is expressed in various tissues including the spleen thymus and liver. FAM111A possesses a serine protease domain which links it to protease activity. Its expression is especially noted in actively proliferating cells suggesting a function in cell cycle regulation.
Biological function summary

FAM111A participates in DNA replication and cell division. It often associates with components of the DNA replication machinery acting to maintain genomic stability during the cell cycle. Researchers have identified FAM111A as an essential factor for proper DNA synthesis. The protein may also interact with other cellular factors to ensure accurate DNA replication although it does not appear to form part of a large stable complex.

Pathways

Studies connect FAM111A to the DNA replication and repair pathways. It interacts with proteins involved in maintaining the integrity of the genome such as those in the replication fork machinery. FAM111A participates alongside proteins like DNA polymerase and helicase where it contributes to their regulation and function. This involvement places FAM111A as an important component for effective DNA damage response and repair.

Mutations in FAM111A are linked to Kenny-Caffey Syndrome and Gracile Bone Dysplasia. These relate to abnormal growth and skeletal development. The protein's dysfunction disrupts normal cell cycle and growth regulator mechanisms. Alterations affecting FAM111A may also involve interactions with cyclins or cyclin-dependent kinases important for proper cell cycle progression. Understanding FAM111A's role can provide insights into therapeutic approaches for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-binding serine protease that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed : 32165630). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed : 32165630). Protects replication fork from stalling by removing DPCs, such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesion, or poly(ADP-ribose) polymerase 1 (PARP1)-DNA complexes trapped by PARP inhibitors (PubMed : 32165630). Required for PCNA loading on replication sites (PubMed : 24561620). Promotes S-phase entry and DNA synthesis (PubMed : 24561620). Acts also as a restriction factor for some viruses including SV40 polyomavirus and vaccinia virus (PubMed : 23093934, PubMed : 37607234). Mechanistically, affects nuclear barrier function during viral replication by mediating the disruption of the nuclear pore complex (NPC) via its protease activity (PubMed : 33369867, PubMed : 37607234). In turn, interacts with vaccinia virus DNA-binding protein OPG079 in the cytoplasm and promotes its degradation without the need of its protease activity but through autophagy (PubMed : 37607234).
See full target information FAM111A
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed39932783

2025

Quantitative hypermorphic FAM111A alleles cause autosomal recessive Kenny-Caffey syndrome type 2 and osteocraniostenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Li,Niels Mailand,Emma Ewing,Saskia Hoffmann,Richard C Caswell,Lewis Pang,Jacqueline Eason,Ying Dou,Kathleen E Sullivan,Hakon Hakonarson,Michael A Levine

The Journal of biological chemistry 301:108175 PubMed39798873

2025

The viral serpin SPI-1 directly inhibits the host cell serine protease FAM111A.

Applications

Unspecified application

Species

Unspecified reactive species

Allison L Welter,Sowmiya Palani,Yuka Machida,Matthew J Schellenberg,Yuichi J Machida

Nature communications 15:2064 PubMed38453899

2024

Dimerization-dependent serine protease activity of FAM111A prevents replication fork stalling at topoisomerase 1 cleavage complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Sowmiya Palani,Yuka Machida,Julia R Alvey,Vandana Mishra,Allison L Welter,Gaofeng Cui,Benoît Bragantini,Maria Victoria Botuyan,Anh T Q Cong,Georges Mer,Matthew J Schellenberg,Yuichi J Machida

Life science alliance 6: PubMed37793778

2023

FAM111A regulates replication origin activation and cell fitness.

Applications

Unspecified application

Species

Unspecified reactive species

Diana O Rios-Szwed,Vanesa Alvarez,Luis Sanchez-Pulido,Elisa Garcia-Wilson,Hao Jiang,Susanne Bandau,Angus Lamond,Constance Alabert

Molecular medicine reports 25: PubMed34796909

2021

Positive feedback loop of lncRNA FAM201A/miR‑146a‑5p/POU2F1 regulates IL‑1β‑induced chondrocyte injury .

Applications

Unspecified application

Species

Unspecified reactive species

Kaifeng Gan,Wei Wu,Jie Li,Dingli Xu,Yunpeng Liu,Mingguang Bi,Liangjie Lu,Jin Li

EMBO reports 22:e50803 PubMed33369867

2020

FAM111A induces nuclear dysfunction in disease and viral restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Minghua Nie,Martina Oravcová,Yasaman Jami-Alahmadi,James A Wohlschlegel,Eros Lazzerini-Denchi,Michael N Boddy

EMBO reports 21:e50662 PubMed32776417

2020

FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Saskia Hoffmann,Satyakrishna Pentakota,Andreas Mund,Peter Haahr,Fabian Coscia,Marta Gallo,Matthias Mann,Nicholas Mi Taylor,Niels Mailand

Nature communications 11:1318 PubMed32165630

2020

FAM111A protects replication forks from protein obstacles via its trypsin-like domain.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Kojima,Yuka Machida,Sowmiya Palani,Thomas R Caulfield,Evette S Radisky,Scott H Kaufmann,Yuichi J Machida

Journal of virology 93: PubMed30333173

2018

Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Roxana M Tarnita,Adrian R Wilkie,James A DeCaprio

Proceedings of the National Academy of Sciences of the United States of America 114:3720-3725 PubMed28320935

2017

Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Debasis Panda,Daniel J Fernandez,Madhu Lal,Eugen Buehler,Bernard Moss
View all publications
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Product promise

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