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AB236132

Anti-FAM111A antibody [EPR14407] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal FAM111A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA1895, FAM111A, Serine protease FAM111A

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] - BSA and Azide free (AB236132)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] - BSA and Azide free (AB236132)

Immunofluorescent analysis of 4% paraformaldehyde-fixed T47-D cells labeling FAM111A with ab184572 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/1200 dilution (red). Counter stained with Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184572).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14407

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab236132 is the carrier-free version of ab184572.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM111A also known as Family with Sequence Similarity 111 Member A is a protein with a molecular mass of approximately 66 kDa. It is expressed in various tissues including the spleen thymus and liver. FAM111A possesses a serine protease domain which links it to protease activity. Its expression is especially noted in actively proliferating cells suggesting a function in cell cycle regulation.
Biological function summary

FAM111A participates in DNA replication and cell division. It often associates with components of the DNA replication machinery acting to maintain genomic stability during the cell cycle. Researchers have identified FAM111A as an essential factor for proper DNA synthesis. The protein may also interact with other cellular factors to ensure accurate DNA replication although it does not appear to form part of a large stable complex.

Pathways

Studies connect FAM111A to the DNA replication and repair pathways. It interacts with proteins involved in maintaining the integrity of the genome such as those in the replication fork machinery. FAM111A participates alongside proteins like DNA polymerase and helicase where it contributes to their regulation and function. This involvement places FAM111A as an important component for effective DNA damage response and repair.

Mutations in FAM111A are linked to Kenny-Caffey Syndrome and Gracile Bone Dysplasia. These relate to abnormal growth and skeletal development. The protein's dysfunction disrupts normal cell cycle and growth regulator mechanisms. Alterations affecting FAM111A may also involve interactions with cyclins or cyclin-dependent kinases important for proper cell cycle progression. Understanding FAM111A's role can provide insights into therapeutic approaches for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-binding serine protease that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed : 32165630). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed : 32165630). Protects replication fork from stalling by removing DPCs, such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesion, or poly(ADP-ribose) polymerase 1 (PARP1)-DNA complexes trapped by PARP inhibitors (PubMed : 32165630). Required for PCNA loading on replication sites (PubMed : 24561620). Promotes S-phase entry and DNA synthesis (PubMed : 24561620). Acts also as a restriction factor for some viruses including SV40 polyomavirus and vaccinia virus (PubMed : 23093934, PubMed : 37607234). Mechanistically, affects nuclear barrier function during viral replication by mediating the disruption of the nuclear pore complex (NPC) via its protease activity (PubMed : 33369867, PubMed : 37607234). In turn, interacts with vaccinia virus DNA-binding protein OPG079 in the cytoplasm and promotes its degradation without the need of its protease activity but through autophagy (PubMed : 37607234).
See full target information FAM111A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Inflammatory bowel diseases 30:2259-2270 PubMed38944815

2024

Solute Transporter OCTN1/Slc22a4 Affects Disease Severity and Response to Infliximab in Experimental Colitis: Role of Gut Microbiota and Immune Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Del Chierico,Letizia Masi,Valentina Petito,Valerio Baldelli,Pierluigi Puca,Roberta Benvenuto,Marco Fidaleo,Ivana Palucci,Loris Riccardo Lopetuso,Maria Emiliana Caristo,Cinzia Carrozza,Maria Cristina Giustiniani,Noritaka Nakamichi,Yukio Kato,Lorenza Putignani,Antonio Gasbarrini,Giovambattista Pani,Franco Scaldaferri

Molecular neurobiology 60:672-686 PubMed36357613

2022

Mito-TEMPO, a Mitochondria-Targeted Antioxidant, Improves Cognitive Dysfunction due to Hypoglycemia: an Association with Reduced Pericyte Loss and Blood-Brain Barrier Leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Lin,Zhou Chen,Cuihua Huang,Yubin Wu,Lishan Huang,Lijing Wang,Sujie Ke,Libin Liu
View all publications
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Product promise

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