AB200718

Anti-FAM111A antibody - N-terminal

Name: Anti-FAM111A antibody - N-terminal (ab200718) | Abcam
Brand: Abcam Limited
SKU: AB200718
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal FAM111A antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FAM111A aa 1-50.

View Alternative Names

KIAA1895, FAM111A, Serine protease FAM111A

1 Images

Supplier Data

Western blot - Anti-FAM111A antibody - N-terminal (AB200718)

All lanes:

Western blot - Anti-FAM111A antibody - N-terminal (ab200718)

Lane 1:

DLD whole cell lysate

Lane 2:

MCF7 whole cell lysate

Lane 3:

A549 whole cell lysate

Lane 4:

RAW 264.7 whole cell lysate

Lane 5:

Sp20 whole cell lysate

Lane 6:

PC12 whole cell lysate

Lane 7:

H9C2 whole cell lysate

Predicted band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human FAM111A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96PZ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM111A also known as Family with Sequence Similarity 111 Member A is a protein with a molecular mass of approximately 66 kDa. It is expressed in various tissues including the spleen thymus and liver. FAM111A possesses a serine protease domain which links it to protease activity. Its expression is especially noted in actively proliferating cells suggesting a function in cell cycle regulation.

Biological function summary

FAM111A participates in DNA replication and cell division. It often associates with components of the DNA replication machinery acting to maintain genomic stability during the cell cycle. Researchers have identified FAM111A as an essential factor for proper DNA synthesis. The protein may also interact with other cellular factors to ensure accurate DNA replication although it does not appear to form part of a large stable complex.

Pathways

Studies connect FAM111A to the DNA replication and repair pathways. It interacts with proteins involved in maintaining the integrity of the genome such as those in the replication fork machinery. FAM111A participates alongside proteins like DNA polymerase and helicase where it contributes to their regulation and function. This involvement places FAM111A as an important component for effective DNA damage response and repair.

Mutations in FAM111A are linked to Kenny-Caffey Syndrome and Gracile Bone Dysplasia. These relate to abnormal growth and skeletal development. The protein's dysfunction disrupts normal cell cycle and growth regulator mechanisms. Alterations affecting FAM111A may also involve interactions with cyclins or cyclin-dependent kinases important for proper cell cycle progression. Understanding FAM111A's role can provide insights into therapeutic approaches for managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Single-stranded DNA-binding serine protease that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed : 32165630). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed : 32165630). Protects replication fork from stalling by removing DPCs, such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesion, or poly(ADP-ribose) polymerase 1 (PARP1)-DNA complexes trapped by PARP inhibitors (PubMed : 32165630). Required for PCNA loading on replication sites (PubMed : 24561620). Promotes S-phase entry and DNA synthesis (PubMed : 24561620). Acts also as a restriction factor for some viruses including SV40 polyomavirus and vaccinia virus (PubMed : 23093934, PubMed : 37607234). Mechanistically, affects nuclear barrier function during viral replication by mediating the disruption of the nuclear pore complex (NPC) via its protease activity (PubMed : 33369867, PubMed : 37607234). In turn, interacts with vaccinia virus DNA-binding protein OPG079 in the cytoplasm and promotes its degradation without the need of its protease activity but through autophagy (PubMed : 37607234).

See full target information FAM111A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39996749

2025

Transcriptomic Insights into Gas6-Induced Placental Dysfunction: Gene Targets for Preeclampsia Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Jackson,Trenton M Gibson,Ethan Frank,Garrett Hill,Benjamin Davidson,Paul R Reynolds,Benjamin T Bikman,Brett E Pickett,Juan A Arroyo

Nature communications 14:5796 PubMed37723159

2023

Discovery of the major 15-30 nt mammalian small RNAs, their biogenesis and function.

Applications

Unspecified application

Species

Unspecified reactive species

Hejin Lai,Ning Feng,Qiwei Zhai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FAM111A antibody - N-terminal

Western blot - Anti-FAM111A antibody - N-terminal (AB200718)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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