AB156695

Anti-FAM120A/OSSA antibody

Name: Anti-FAM120A/OSSA antibody (ab156695) | Abcam
Brand: Abcam Limited
SKU: AB156695
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal FAM120A/OSSA antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human FAM120A aa 1050 to C-terminus.

View Alternative Names

C9orf10, KIAA0183, OSSA, FAM120A, Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated SRC activator, Protein FAM120A

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Immunoprecipitation - Anti-FAM120A/OSSA antibody (AB156695)

Detection of FAM120A/OSSA by Western blot of immunoprecipitate.
Immunoprecipitation of FAM120A/OSSA (6 μg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following :
Lane 1 : alternative rabbit anti-FAM120A/OSSA antibody
Lane 2 : ab156695
Lane 3 : Control IgG
For the subsequent Western blot of immunoprecipitates, ab156695 was used at 1 μg/ml.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FAM120A/OSSA antibody (ab156695)

Predicted band size: 121 kDa

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Western blot - Anti-FAM120A/OSSA antibody (AB156695)

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-FAM120A/OSSA antibody (ab156695) at 1 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 121 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human FAM120A aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZB2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab156695 was affinity purified using an epitope specific to FAM120A/OSSA immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM120A also known as OSSA is a protein that plays a role in cellular signaling. It has a mass of approximately 120 kDa. Researchers observe the expression of this protein in various tissues including testis ovary and brain. FAM120A functions as a scaffolding protein which means it helps organize other proteins at specific locations within cells to facilitate interactions and signaling pathways.

Biological function summary

FAM120A interacts with numerous cellular components and exhibits roles in regulating stress response. It integrates into the RNA granule complex allowing it to influence RNA stability and translation. Through its interaction with the cytoplasmic polyadenylation element-binding proteins it modulates mRNA processing offering insight into how cells manage protein production in response to environmental changes.

Pathways

FAM120A becomes integrated into signaling cascades like the PI3K/Akt pathway. It influences cell survival and growth by modulating interactions with proteins such as Src and Grb2. These interactions contribute to the regulation of key signaling activities that impact various cell functions including proliferation and apoptosis.

FAM120A has connections to cancer and cardiomyopathies. Alterations in its expression or function may affect disease pathogenesis. For instance its relationship with Src contributes to oncogenic signaling pathways influencing cancer progression. In cardiac diseases this protein interacts with pathways that involve binding partners like PI3K where its dysregulation can lead to aberrant heart function or pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the oxidative stress-induced survival signaling. May regulate the activation of SRC family protein kinases (PubMed : 19015244). May act as a scaffolding protein enabling SRC family protein kinases to phosphorylate and activate PI3-kinase (PubMed : 19015244). Binds IGF2 RNA and promotes the production of IGF2 protein (PubMed : 19015244).

See full target information FAM120A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:13402-13418 PubMed39470700

2024

Systematic analysis of the target recognition and repression by the Pumilio proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Farberov,Igor Ulitsky

Nucleic acids research 52:9917-9935 PubMed38994560

2024

Loss of Lamin A leads to the nuclear translocation of AGO2 and compromised RNA interference.

Applications

Unspecified application

Species

Unspecified reactive species

Vivian Lobo,Iwona Nowak,Carola Fernandez,Ana Iris Correa Muler,Jakub O Westholm,Hsiang-Chi Huang,Ivo Fabrik,Hang T Huynh,Evgeniia Shcherbinina,Melis Poyraz,Anetta Härtlova,Daniel Benhalevy,Davide Angeletti,Aishe A Sarshad

Communications biology 7:399 PubMed38565940

2024

FAM120A deficiency improves resistance to cisplatin in gastric cancer by promoting ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liangbo Niu,Yi Li,Guixiang Huang,Wei Huang,Jing Fu,Lu Feng

Nucleic acids research 46:11575-11591 PubMed30247708

2018

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Applications

Unspecified application

Species

Unspecified reactive species

Evan P Booy,Ewan Ks McRae,Peyman Ezzati,Taegi Choi,Daniel Gussakovsky,Sean A McKenna

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FAM120A/OSSA antibody

Immunoprecipitation - Anti-FAM120A/OSSA antibody (AB156695)

Western blot - Anti-FAM120A/OSSA antibody (AB156695)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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