Rabbit Recombinant Monoclonal RETR1 antibody. Suitable for WB, IP and reacts with Human samples.
Images
![Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (AB316161), expandable thumbnail](https://content.abcam.com/products/images/fam134b-antibody-epr28817-67-ab316161--immunoprecipitation-img426251.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (AB316161)")
![Western blot - Anti-FAM134B antibody [EPR28817-67] (AB316161), expandable thumbnail](https://content.abcam.com/products/images/fam134b-antibody-epr28817-67-ab316161--western-blot-img426250.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAM134B antibody [EPR28817-67] (AB316161)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Endoplasmic reticulum (ER)-anchored autophagy regulator which mediates ER delivery into lysosomes through sequestration into autophagosomes (PubMed:26040720, PubMed:31930741, PubMed:34338405). Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins (PubMed:26040720, PubMed:31930741, PubMed:34338405). Active under basal conditions (PubMed:34338405). Required for collagen quality control in a LIR motif-dependent manner (By similarity). Required for long-term survival of nociceptive and autonomic ganglion neurons (PubMed:19838196, PubMed:26040720). (Microbial infection) During SARS-CoV-2 infection, RETREG1-mediated reticulophagy is promoted by SARS-CoV-2 ORF3A protein (PubMed:35239449). This induces endoplasmic reticulum stress and inflammatory responses and facilitates viral infection (PubMed:35239449).
Alternative names
FAM134B, JK1, RETREG1, Reticulophagy regulator 1, Reticulophagy receptor 1
Recommended products
Rabbit Recombinant Monoclonal RETR1 antibody. Suitable for WB, IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28817-67
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein FAM134B also known as JK-1 is an autophagy regulator with a molecular mass of approximately 50 kDa. FAM134B plays a critical role in the degradation of endoplasmic reticulum (ER) membranes. This protein expresses robustly in the neural tissues gastrointestinal tract and reproductive organs. FAM134B functions by binding to membranes and facilitating the removal of damaged ER components which maintains cellular homeostasis.
- Biological function summary
FAM134B serves as an important player in maintaining cell health by its involvement in ER-phagy a selective autophagy process. It forms part of the ER-phagy complex and works alongside LC3 proteins to ensure efficient turnover of the ER membrane. Its activity helps prevent ER stress and safeguard cell survival under stress conditions. Failure in this pathway can lead to accumulation of misfolded proteins and impaired cell function.
- Pathways
FAM134B interacts in the process of ER-associated degradation (ERAD) and autophagy-related pathways. It participates importantly in the calcium signaling pathway where FAM134B affects the homeostasis of calcium levels in the cytoplasm and ER. This interaction involves key signaling proteins such as ATG8 and ER-phagy receptors showing its essential role in cellular adaptation to stress.
- Associated diseases and disorders
FAM134B has significant associations with sensory neuropathy and colorectal cancer. In sensory neuropathy mutations or dysfunctions in FAM134B lead to the loss of pain perception due to improper ER turnover in neurons. It also links to colorectal cancer often showing altered expression that affects tumor progression. FAM134B interacts with proteins like p53 in cancer pathways which influences cell cycle regulation and apoptosis in cancerous cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (ab316161), expandable thumbnail](https://content.abcam.com/products/images/fam134b-antibody-epr28817-67-ab316161--immunoprecipitation-img426251.jpg "Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (ab316161)")
Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (ab316161)
FAM134B was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab316161 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316161 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab316161 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316161 in HeLa whole cell lysateAll lanes: Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (ab316161) at 1/30 dilution
All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 41s
![Western blot - Anti-FAM134B antibody [EPR28817-67] (ab316161), expandable thumbnail](https://content.abcam.com/products/images/fam134b-antibody-epr28817-67-ab316161--western-blot-img426250.jpg "Western blot - Anti-FAM134B antibody [EPR28817-67] (ab316161)")
Western blot - Anti-FAM134B antibody [EPR28817-67] (ab316161)
Low expression: HCT116(PMID: 28549913)
Lane 1 was freshly made and used for Western blotting immediately to minimize protein degradation.
Lane 2-6 were developed using a higher sensitivity ECL substrate.
Samples are non-boiled as boiling may cause protein aggregation.
The bands beneath the target band (70 kDa) are expected to be degradation products.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 180 seconds; Lane 2: 81 seconds; Lane 3-6: 180 seconds.
All lanes: Western blot - Anti-FAM134B antibody [EPR28817-67] (ab316161) at 1/1000 dilution
Lanes 1 and 3: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 5: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 6: HeLa transfected with siRNA specifically targeting FAM134B whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50 kDa, 60 kDa, 70 kDa, 36 kDa
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