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AB316161

Anti-FAM134B antibody [EPR28817-67]

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Rabbit Recombinant Monoclonal RETR1 antibody. Suitable for WB, IP and reacts with Human samples.

View Alternative Names

FAM134B, JK1, RETREG1, Reticulophagy regulator 1, Reticulophagy receptor 1

2 Images
Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (AB316161)
  • IP

Supplier Data

Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (AB316161)

FAM134B  was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab316161 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316161 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab316161 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316161 in HeLa whole cell lysate

All lanes:

Immunoprecipitation - Anti-FAM134B antibody [EPR28817-67] (ab316161) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

Western blot - Anti-FAM134B antibody [EPR28817-67] (AB316161)
  • WB

Supplier Data

Western blot - Anti-FAM134B antibody [EPR28817-67] (AB316161)

Low expression : HCT116(PMID : 28549913)

Lane 1 was freshly made and used for Western blotting immediately to minimize protein degradation.

Lane 2-6 were developed using a higher sensitivity ECL substrate.

Samples are non-boiled as boiling may cause protein aggregation.

The bands beneath the target band (70 kDa) are expected to be degradation products.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 180 seconds; Lane 2 : 81 seconds; Lane 3-6 : 180 seconds.

All lanes:

Western blot - Anti-FAM134B antibody [EPR28817-67] (ab316161) at 1/1000 dilution

Lanes 1 and 3:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 4:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 6:

HeLa transfected with siRNA specifically targeting FAM134B whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,60 kDa,70 kDa,36 kDa

false

  • Carrier free

    Anti-FAM134B antibody [EPR28817-67] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28817-67

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FAM134B also known as JK-1 is an autophagy regulator with a molecular mass of approximately 50 kDa. FAM134B plays a critical role in the degradation of endoplasmic reticulum (ER) membranes. This protein expresses robustly in the neural tissues gastrointestinal tract and reproductive organs. FAM134B functions by binding to membranes and facilitating the removal of damaged ER components which maintains cellular homeostasis.
Biological function summary

FAM134B serves as an important player in maintaining cell health by its involvement in ER-phagy a selective autophagy process. It forms part of the ER-phagy complex and works alongside LC3 proteins to ensure efficient turnover of the ER membrane. Its activity helps prevent ER stress and safeguard cell survival under stress conditions. Failure in this pathway can lead to accumulation of misfolded proteins and impaired cell function.

Pathways

FAM134B interacts in the process of ER-associated degradation (ERAD) and autophagy-related pathways. It participates importantly in the calcium signaling pathway where FAM134B affects the homeostasis of calcium levels in the cytoplasm and ER. This interaction involves key signaling proteins such as ATG8 and ER-phagy receptors showing its essential role in cellular adaptation to stress.

FAM134B has significant associations with sensory neuropathy and colorectal cancer. In sensory neuropathy mutations or dysfunctions in FAM134B lead to the loss of pain perception due to improper ER turnover in neurons. It also links to colorectal cancer often showing altered expression that affects tumor progression. FAM134B interacts with proteins like p53 in cancer pathways which influences cell cycle regulation and apoptosis in cancerous cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum (ER)-anchored autophagy regulator which mediates ER delivery into lysosomes through sequestration into autophagosomes (PubMed : 26040720, PubMed : 31930741, PubMed : 34338405). Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins (PubMed : 26040720, PubMed : 31930741, PubMed : 34338405). Active under basal conditions (PubMed : 34338405). Required for collagen quality control in a LIR motif-dependent manner (By similarity). Required for long-term survival of nociceptive and autonomic ganglion neurons (PubMed : 19838196, PubMed : 26040720).. (Microbial infection) During SARS-CoV-2 infection, RETREG1-mediated reticulophagy is promoted by SARS-CoV-2 ORF3A protein (PubMed : 35239449). This induces endoplasmic reticulum stress and inflammatory responses and facilitates viral infection (PubMed : 35239449).
See full target information RETREG1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com